Project description:Paenarthrobacter nicotinovorans pAO1 is a nicotine degrading microorganism that shows promising applications in converting nicotine-containing waste into useful green chemicals. Its biotechnological applications are nevertheless hampered by the lack of knowledge and tools to perform genetic and metabolic engineering. The objective of the work is to provide the first transcriptome of the strain and is a second step in our envisioned complete omics characterization of nicotine metabolism in P. nicotinovorans ATCC 49919. Acknowledgements. This work was supported by a grant of the Romanian Ministry of Education and Research, CNCS - UEFISCDI, project number PN-III-P4-ID-PCE-2020-0656, within PNCDI III.
Project description:The shotgun proteomics approach attempts to identify the proteins expressed by Paenarthrobacter (formerly Arthrobacter) nicotinovorans in the presence of nicotine by using nanoLCMSMS. A total of 792 non redundant proteins were identified when P. nicotinovorans was grown in on citrate, nicotine and citrate and nicotine as the only carbon source.
Project description:We used nanoLC–MS/MS in a shotgun proteomics approach in order to identify the proteins expressed by P. nicotinovorans in the presence of nicotine at 3 different time intervals: 7, 10 and 24 hours post inoculation. A total of 915 proteins grouped 584 non-redundant clusters in were identified with an FDR of 0.3% when P. nicotinovorans was grown in on citrate medium alone and on citrate supplemented 0.05% nicotine. The differences in protein expression pattens at different time intervals allows us have a more dynamic picture on how the nicotine catabolic pathway is activated and to related it to the appearance of the known metabolic intermediates.
