Project description:We cultured bone marrow derived dendritic cells from WT and CD11c KO mice. Then, a group of bone marrow dendritic cells were stimulated with LPS overnight. We obtained bone marrow derived dendritic cells with or without LPS stimulation and analyzed proteomics profiles.
Project description:ATAC-seq profiling of Nfat5 KO and wild type macrophages derived from bone marrow (primary cells), treated or not with Lipopolysaccharide (LPS).
Project description:Dendritic cells isolated from murine bone marrow were cultured for 7 days and then stimulated with 100 ng/mL LPS for 24h. Cells were derived from either wildtype or microRNA-146a knock-out mice. Total RNA was isolated from the cells following stimulation.
Project description:Dendritic cells isolated from murine bone marrow were cultured for 7 days and then stimulated with 50 ng/mL LPS and 5 mM ATP. Cells derived from both wild type and microRNA-155 knock-out mice. Total RNA was isolated from the cells following stimulation.
Project description:To comprehensively understand how dendritic cells (DCs) are reprogrammed by lung fibroblasts- and their derived COX-2/PGE2, we employed lung fibroblasts isolated from WT or Ptgs2-/- mice, and collect their conditioned medium (CM) to stimulate the ex vivo cultured bone marrow (BM)-derived DCs (BM-DCs), with the PGE2 treatment as a control. After the treatment, BM-DCs were harvested for RNA extraction and the transcriptional profiles were analyzed by RNA sequencing (RNA-seq).
