Project description:Expression profiles of polg mutant cells reveal that many genes encoding proteins involved in cell wall biogenesis and stress response are induced, suggesting that polg mutant cells attempt to maintain growth potential and undergo extensive oxidative metabolism. Conversely, many genes encoding proteins involved in ribosome biogenesis and respiration are repressed, indicating that cells depleted of mtDNA are adapted to grow slowly in absence of mitochondrial function. Keywords: Schizosaccharomyces pombe ployg mutant cells vs wild type cells.
Project description:Puf3 is a RNA-binding protein, a member of the conserved Puf-protein family. Combining different functional genomics data, we have analyzed the role of Puf3 in post-transcriptional gene regulation in S. pombe. We present data on Puf3 interacting proteins and regulatory mRNA targets.
Project description:Aging is a highly regulated process in eukaryotes. Many biochemical pathways are affected by aging and, among them, protein homeostasis is one key process. Our hypothesis is that protein translation may be important to maintain a correct balance of active, properly folded proteins in the cell and, therefore, help supporting a normal longevity. Phosphorylation of eIF2alpha is one of the most important regulatory steps in protein translation in eukaryotes, reducing total protein translation and promoting the specific production of some proteins (Gcn4, Atf4, etc) We decided to test whether eIF2alpha phosphorylation has specific roles in protein translation during aging in Schizosaccharomyces pombe
Project description:Hrp3_Purification from Schizosaccharomyces pombe 972h- Eukaryotic genome is composed of repeating units of nucleosomes to form chromatin arrays. A canonical gene is marked by nucleosome free region (NFR) at its 5’ end followed by uniformly spaced arrays of nucleosomes. In fission yeast we show both biochemically and in vivo that both Hrp1 and Hrp3 are key determinants of uniform spacing of genic arrays.
