Project description:Survey of relative gene expression profiles in larvae, pupae, and midguts and fatbodies of teneral, unfed, and fed adult mountain pine beetles from western Nevada, USA. Array prepared on 4-tile chips (4-plex custom arrays), each feature represented by six different oligonucleotides, three replicate blocks/tile, hybridized with total RNA from 11 samples, each replicated four times. Vector (pDONR222) and two plant sequences included as negative controls.
Project description:The presence of DNA methylation in beetles (Coleoptera) has only been investigated with bisulfite sequencing of Tribolium castaneum, which produced no evidence of DNA methylation. Here, we used whole genome bisulfite sequencing to assay if DNA methylation was present in another beetle, Nicrophorus vespilloides. We used T. castaneum as a negative control.
