Marine metagenome
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REPOSITORIES: ENA
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Project description:In this study, we examined the transcriptomic responses to temperature acclimation (14oC, 20oC, and 25oC) in atrial and ventricular tissues of Pacific bluefin tuna (PBFT). A global gene expression analysis using a bluefin tuna-specific microarray indicated profound changes in expression of genes associated with energy metabolism, protein turnover, cellular stress response, oxidative stress and apoptosis. A principal component analysis revealed tissue-specific transcriptomic responses to temperature, with atrium at 25oC showing the greatest variation. Overall transcriptomic data suggests that PBFT can optimize cardiac function in the cold by acclimating to 14oC. Capacity to acclimate to colder temperatures potentially underlies this species ability to expand its vertical and horizontal thermal niche and migrate to colder oceans at high latitudes. In contrast, the cardiac phenotype of 25oC acclimated fish infers that PBFT hearts struggle to maintain cellular homeostasis and are subjected to programmed cell death. The goal of this study was to compare transcriptomic response to cold and warm temperature acclimations across cardiac tissues in Pacific bluefin tuna. Fish (n=4) were acclimated to 14C, 20C and 25C, and RNA was extracted from atrial, ventricular compact and spongy tissues. Experimental samples were hybridyzed against a reference pool that contained a mix of RNA from every sample.
2012-03-31 | E-GEOD-36886 | biostudies-arrayexpress
Project description:In this study, we examined the transcriptomic responses to temperature acclimation (14oC, 20oC, and 25oC) in atrial and ventricular tissues of Pacific bluefin tuna (PBFT). A global gene expression analysis using a bluefin tuna-specific microarray indicated profound changes in expression of genes associated with energy metabolism, protein turnover, cellular stress response, oxidative stress and apoptosis. A principal component analysis revealed tissue-specific transcriptomic responses to temperature, with atrium at 25oC showing the greatest variation. Overall transcriptomic data suggests that PBFT can optimize cardiac function in the cold by acclimating to 14oC. Capacity to acclimate to colder temperatures potentially underlies this species ability to expand its vertical and horizontal thermal niche and migrate to colder oceans at high latitudes. In contrast, the cardiac phenotype of 25oC acclimated fish infers that PBFT hearts struggle to maintain cellular homeostasis and are subjected to programmed cell death.
2012-04-01 | GSE36886 | GEO
Project description:Diet analysis of Japanese sardine and Pacific round herring larvae
| PRJDB4816 | ENA
Project description:Genome assembly of the Pacific bluefin tuna and re-sequence data analysis
| PRJDB8174 | ENA
Project description:The largest of the tuna species, Atlantic bluefin tuna, Thunnus thynnus (Linnaeus, 1758), inhabits the North Atlantic Ocean and the Mediterranean Sea and is considered to be an endangered species, largely through overfishing. Thus, the development of aquaculture practices independent of wild resources can provide an important contribution towards ensuring security and sustainability of this species in the longer-term. In order to provide a resource for ongoing studies, we have used 454 pyrosequencing technology to sequence a mixed-tissue normalized cDNA library, derived from adult individuals. Transcript sequences were used to develop a novel 15K Agilent oligo microarray for T. thynnus and comparative tissue gene expression profiles were inferred for gill, heart, liver, ovaries and testes.
2015-08-10 | E-MTAB-3412 | biostudies-arrayexpress
Project description:We produced differentially sensitive-temperature phenotypes using genetically defined larval families of the bivalve Crassostrea gigas. Larval growth rates varied ~5-fold and reciprocal hybrids showed different genotype-dependent responses over a 15-25°C temperature range. Whole-genome expression analysis of ~24 million cDNAs from larvae identified 22,250 unique transcripts. Of these, ~15% showed a significant interaction between genotype and temperature and are associated with genotype-dependent differences in response to temperature. Examination of 2 genotypes of Pacific oyster larvae, grown at 3 temperatures
2011-08-01 | E-GEOD-13028 | biostudies-arrayexpress
Project description:Thunnus maccoyii (Southern bluefin tuna) genome assembly, fThuMac1
| PRJEB46021 | ENA