Project description:To elucidate the mechanisms of fruit body development in Pleurotus ostreatus, the transcriptomes of four different development stages including mycelium, primordium, young fruit body, and mature fruit body were obtained by RNA-seq.
Project description:Pleurotus tuoliensis is a precious edible fungus with extremely high nutritive and medicinal value. The cultivation period of P. tuoliensis is longer than those of other Pleurotus species, which is mainly due to a longer mycelium physiological maturation period (30-60 days). Currently, the molecular processes underlying physiological maturation of the mycelium remain unclear. We performed a comparative transcriptomic analysis of immature and mature mycelia using RNA-seq. De novo transcriptome assembly resulted in identification of 17,030 unigenes. 451 differentially expressed genes, including those encoding nucleoside diphosphate kinase (NDPK), glycoside hydrolase family proteins, exopolygalacturonase, and versatile peroxidases, were identified. GO and KEGG analyses revealed that nucleotide synthesis and energy metabolism are highly active during the physiological maturation of mycelia, and genes related to these pathways were significantly up-regulated in mature mycelia. NDPK is predicted to be essential for mycelia maturation. Our findings contribute to a comprehensive understanding of mycelia maturation in a commercially important fungal species. Future efforts will focus on the function of NDPK and the mechanism by which it regulates mycelia maturation.
Project description:Pleurotus ostreatus, also known as the oyster mushroom, is an active lignin decomposer in the forests. The genomes of the monokaryotic strains PC15 and PC9 have been used to characterize the content and distribution of transposable elements. This study analyzes the impact of transposable element insertions on the global transcriptome of P. ostreatus. The transcriptome of PC15 and PC9 has been analyzed in exponential growth during submerged fermentation in malt-yeast extract-sucrose medium RNAseq of two P. ostreatus strains: PC15 and PC9
Project description:Hypsizygus marmoreus is one of the most popular industrialized cultivation mushrooms in East Asia. H. marmoreus is the third wildly cultivated industrialized cultivated mushroom in China now behind only Flammulina velutipes and Pleurotus eryngii. H. marmoreus is a low-temperature fruiting mushroom. The mycelia grow vegetatively at 23 °C to 25 °C and transform into fruiting process at 13 °C to 17 °C. The fruiting process of H. marmoreus is sensitive to temperature, and high temperature can lead to the death of mycelia and malformation of the fruiting body. Therefore, thermotolerance is important for H. marmoreus during both vegetative growth and fruiting-body formation. Previous studies on H. marmoreus have mainly focused on cultivation, nutrition and bioactive compounds extracted, but little is known about thermotolerance. The aim of this project is to elucidate the thermotolerance mechanism of Hypsizygus marmoreus.
Project description:Pleurotus ostreatus, also known as the oyster mushroom, is an active lignin decomposer in the forests. The genomes of the monokaryotic strains PC15 and PC9 have been used to characterize the content and distribution of transposable elements. This study analyzes the impact of transposable element insertions on the global transcriptome of P. ostreatus. The transcriptome of PC15 and PC9 has been analyzed in exponential growth during submerged fermentation in malt-yeast extract-sucrose medium
Project description:The whole-genome gene expression profiling was performed by RNA-Seq after 96 h HS treatment of P. tuoliensis mycelia. Divergent mycelial growth rates were observed under the HS with different intensities, which facilitated comparative analyses combining both physiological and transcriptomic data. The goal aimed to acquire a deeper insight into the genome-wide gene expression patterns during the response to HS in mushrooms.
Project description:Transcriptomes of Pleurotus ostreatus in four different development stages (mycelium, primordium, young fruit body, mature fruit body)
Project description:Photoresponse mechanism of oyster muchroom mycelia was studied using a custom microarray prepared on the basis of the genome information (Pleurotus ostreatus PC15 v2.0) in JGI Genome Portal. Blue light stimulation to the mycelia caused the up-regulation and down-regulation of genes expression. Particulary, the genes coding rate-controlling enzymes in glycolysis, pentose phosphate, and sikimic acid pathways were up-regulated to accumulate shikimic acid dramatically.
