ABSTRACT: Environmental metagenomics, soil fungal and prokaryotic community in three black locust forests along an aridity gradient of Loess Plateau, China
Project description:Environmental metagenomics, soil fungal and prokaryotic communities in black locust forests and oak forests along an aridity gradient of Loess Plateau, China
| PRJDB8422 | ENA
Project description:Environmental metagenomics, Soil fungal and prokaryotic communities in black locust forests and oak forests of Loess Plateau, China
Project description:Higher aridity and more extreme rainfall events in drylands are predicted under climate change. Yet it is unclear how changing precipitation regimes may affect nitrogen (N) cycling, especially in areas with extremely high aridity. Here we investigated soil N isotopic values (M-NM-415N) along a 3200 km aridity gradient and show a hump-shaped relationship between soil M-NM-415N and aridity index (AI) with a threshold at AI=0.32. Also, using a micro-array metageomics tool named GeoChip 5.0, we showed that Variations of nitrification and denitrification gene abundance along the gradient which provide further evidence for the existence of this threshold. Data support the hypothesis that the increase of gaseous N losses is higher than the increase of net plant N accumulation with increasing AI below AI=0.32, while the opposite is favoured above this threshold. Our results suggest the importance of N-cycling microbes in extremely dry areas and the different controlling factors of N cycling on the either side of the threshold.
Project description:Environmental metagenomics, arbuscular mycorrhizal fungi in tree roots in a black locust forest and oak forest of Loess Plateau, China
Project description:Amplicon sequencing of environmental DNA, Soil fungal and prokaryotic communities under an artificial rainfall experiment in a black locust forest and an oak forest of Loess Plateau, China
Project description:Anthropogenic nitrogen (N) deposition may affect soil organic carbon (SOC) decomposition, thus affecting the global terrestrial carbon (C) cycle. However, it remains unclear how the level of N deposition affects SOC decomposition by regulating microbial community composition and function, especially C-cycling functional genes structure. We investigated the effects of short-term N addition on soil microbial C-cycling functional gene composition, SOC-degrading enzyme activities, and CO2 emission in a 5-year field experiment established in an artificial Pinus tabulaeformis forest on the Loess Plateau, China.
