Phyllosphere metagenome
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REPOSITORIES: ENA
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Project description:<p><strong>BACKGROUND:</strong> Modern biological approaches generate volumes of multi-dimensional data, offering unprecedented opportunities to address biological questions previously beyond reach owing to small or subtle effects. A fundamental question in plant biology is the extent to which below-ground activity in the root system influences above-ground phenotypes expressed in the shoot system. Grafting, an ancient horticultural practice that fuses the root system of one individual (the rootstock) with the shoot system of a second, genetically distinct individual (the scion), is a powerful experimental system to understand below-ground effects on above-ground phenotypes. Previous studies on grafted grapevines have detected rootstock influence on scion phenotypes including physiology and berry chemistry. However, the extent of the rootstock's influence on leaves, the photosynthetic engines of the vine, and how those effects change over the course of a growing season, are still largely unknown.</p><p><strong>RESULTS:</strong> Here, we investigate associations between rootstock genotype and shoot system phenotypes using 5 multi-dimensional leaf phenotyping modalities measured in a common grafted scion: ionomics, metabolomics, transcriptomics, morphometrics, and physiology. Rootstock influence is ubiquitous but subtle across modalities, with the strongest signature of rootstock observed in the leaf ionome. Moreover, we find that the extent of rootstock influence on scion phenotypes and patterns of phenomic covariation are highly dynamic across the season.</p><p><strong>CONCLUSIONS:</strong> These findings substantially expand previously identified patterns to demonstrate that rootstock influence on scion phenotypes is complex and dynamic and underscore that broad understanding necessitates volumes of multi-dimensional data previously unmet.</p>
2022-01-07 | MTBLS2831 | MetaboLights
Project description:Grassland above and below ground community shift across soil moisture transition
| PRJEB47280 | ENA
Project description:Skin Microbiota Above and Below the Panniculus
| PRJNA305923 | ENA
Project description:Spider communities link above- and below-ground food webs: high-throughput sequencing of Hexapoda prey from spider samples
| PRJDB5193 | ENA
Project description:Plants are colonized by a variety of microorganisms, the plant microbiota. In the phyllosphere, the above-ground parts of plants, bacteria are the most abundant inhabitants. Most of these microorganisms are not pathogenic and the plant responses to commensals or to pathogen infection in the presence of commensals are not well understood. We report the Arabidopsis leaf transcriptome after 3 to 4 weeks of colonization by Methylobacterium extorquens PA1 and Sphingomonas melonis Fr1, representatives of two abundant genera in the phyllosphere, compared to axenic plants. In addition, we also sequenced the transcriptome of Arabidopsis 2 and 7 days after spray-infection with a low dose of P. syringae DC3000 and in combination with the commensals.
2016-06-05 | E-MTAB-4683 | biostudies-arrayexpress
Project description:Comparative gene expression profiling of a) above-ground tissues of three fruiting species (P. cheesemanii, ch, P. exile, ex, P. novae-zelandiae, nz), b) above-ground tissues of two rosette stage species (P. fastigiatum, fa, P. enysii, en) and c) roots of all five species using heterologous A. thaliana microarrays
2009-11-17 | GSE19017 | GEO
Project description:In horticulture, grafting is a popular technique used to combine positive features of two different plants, obtained by joining a scion (generally the part above the ground) onto a rootstock (constituted by the stem and roots). Despite its wide-use, the biological mechanisms driving rootstock-induced alterations of the scion phenotype are not fully understood. Given that epigenetics is an important component of distance signalling in plants, we investigated the genome wide changes in the DNA methylation induced in eggplants grafted onto two interspecific rootstocks commercially used to induce scion vigour, compared to self-grafted plants. We found that vigour was associated to a specific change in scion gene expression and a genome wide hypo-methylation in CHH context. Interestingly, this hypomethylation correlated with downregulation of younger and potentially more active LTR retrotransposons. Our data indicate that graft-induced epigenetics modifications are associated to both physiological and molecular phenotypes in plants, and suggest that rootstocks can induce vigour by reducing DNA methylation in the scion genome, similarly to what observed in some heterotic hybrids.
2021-09-30 | GSE136784 | GEO