Project description:Chronic kidney disease of uncertain etiology (CKDu) is an increasing problem in Sri Lanka especially among the farming community. The etiologies although uncertain, have been associated with occupational and environmental factors. The expression pattern of genes in blood of these CKDu patients in an endemic region of Sri Lanka was analyzed compared to healthy individuals from a non-endemic region to see any expression changes that could be associated to environmental factors. Pattern of expression changes could lead to either strengthen or weaken current hypotheses for the causes.
Project description:In Asia, oral cancer (OC) and oral submucous fibrosis (OSF) constitute major health problems linked to use of betel quid. This work performed CGH genome-wide analysis of OC (12 from India, 12 from Sri Lanka) and OSF (6 from India) cases with normal controls.
Project description:In order to study the proteome of Echis carinatus venom from Sri Lanka (SL ECV), crude SL ECV was subjected to 12.5% SDS-PAGE analysis. The resulting SDS-PAGE bands were subjected to in-gel trypsin digestion and subsequent tandem mass spectrometry analysis. The raw data were then analysed using PEAKS 8.5 software to finally decipher the complex venom proteome.
Project description:Hypnale hypnale (hump-nosed pit viper) is considered to be one among the medically important venomous snake species of India and Sri Lanka. In the present study, venom proteome profiling of a single Hypnale hypnale from Western Ghats of India was achieved using SDS-PAGE based protein separation followed by LC-MS/MS analysis
