Leishmania braziliensis MHOM/BR/75/M2904
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ABSTRACT:
PROVIDER: PRJEA18663 | ENA |
REPOSITORIES: ENA
Similar Datasets
Project description:The main etiological agent of South American cutaneous leishmaniasis (ACL)
| PRJNA19183 | ENA
Project description:Leishmania braziliensis MHOM/BR/75/M2903 Genome sequencing and assembly
| PRJNA165955 | ENA
Project description:Leishmania (Viannia) braziliensis is the main etiological agent of cutaneous and mucocutaneous leishmaniasis in Latin America. Reports have described non-ulcerated atypical tegumentary leishmaniasis cases caused by L. braziliensis in several regions of the world, including in patients from the Xacriabá Indigenous reserve, in São João das Missões/Minas Gerais - Brazil. Parasites isolated from these atypical clinical lesions have previously been found to be resistant to antimony-based therapeutics. In the present study, proteins displaying differential abundance in 2 strains of L. braziliensis isolated from patients with atypical lesions compared with 4 strains isolated from patients with typical lesions were identified using a quantitative proteomics approach based on tandem mass tag labeling (TMT) and mass spectrometry. A total of 532 (p value <0.05) differentially abundant proteins were identified (298 up-regulated and 234 down-regulated) in strains from atypical lesions compared to strains from typical lesions. We observed a variety of proteins with differential abundance among the studied strains. Prominent positively regulated in atypical strains included proteins which may confer a greater survival inside the macrophage, proteins related to resistance to antimony and higher peroxidase activity. Also were identified proteins suggest as new drug and vaccines target. Our data contribute to characterization of these intriguing L. braziliensis strains, and sheds new light on ACL cases has been associated with therapeutic failures.
2022-02-11 | PXD029995 | Pride
Project description:The host immune response plays a critical role not only in protection from human leishmaniasis, but also in promoting disease severity. Although candidate gene approaches in mouse models of leishmaniasis have been extremely informative, a global understanding of the immune pathways active in lesions from human patients is lacking. To address this issue, genome-wide transcriptional profiling of Leishmania braziliensis-infected cutaneous lesions and normal skin controls was carried out. A signature of the L. braziliensis skin lesion was defined that includes over 2,000 differentially regulated genes. Pathway-level analysis of this transcriptional response revealed key biological pathways, as well as specific genes, associated with cutaneous pathology, generating a testable 'metapathway' model of immune-driven lesion pathology, and providing new insights for treatment of human leishmaniasis. Thirty-five skin biopsies were analyzed, including 10 normal skin biopsies (2 from North America and 8 from non-endemic area in Brazil), and 25 skin lesion biopsies (8 early cutaneous lesions, 17 late cutaneous lesions) obtained from Leishmania brazilensis-infected patients presenting at the Corte de Pedra Health Post in Corte de Pedra, Bahia, Brazil.
2014-03-07 | E-GEOD-55664 | biostudies-arrayexpress
Project description:The host immune response plays a critical role not only in protection from human leishmaniasis, but also in promoting disease severity. Although candidate gene approaches in mouse models of leishmaniasis have been extremely informative, a global understanding of the immune pathways active in lesions from human patients is lacking. To address this issue, genome-wide transcriptional profiling of Leishmania braziliensis-infected cutaneous lesions and normal skin controls was carried out. A signature of the L. braziliensis skin lesion was defined that includes over 2,000 differentially regulated genes. Pathway-level analysis of this transcriptional response revealed key biological pathways, as well as specific genes, associated with cutaneous pathology, generating a testable 'metapathway' model of immune-driven lesion pathology, and providing new insights for treatment of human leishmaniasis.
2014-03-07 | GSE55664 | GEO
Project description:This study was carried out to evaluate the changes that occur in the skin after the development of cutaneous leishmaniasis, aiming at a comprehensive understanding of immune pathways and biological functions activated in lesions caused by L. braziliensis.
2015-02-01 | GSE63931 | GEO
Project description:This study was carried out to evaluate the changes that occur in the skin after the development of cutaneous leishmaniasis, aiming at a comprehensive understanding of immune pathways and biological functions activated in lesions caused by L. braziliensis. This analysis was conducted on 8 skin ulcers from patients infected with L. braziliensis. The patients selected for the gene expression analysis had recent L. braziliensis infection that had not yet been treated. 8 controls samples are skin biopsies from healthy donors (non-infected).
2015-02-01 | E-GEOD-63931 | biostudies-arrayexpress
Project description:A flagellated protozoan that is the etiological agent of cutaneous, mucocutaneous and/or visceral leishmaniasis.
| PRJNA9528 | ENA
Project description:As a vector-borne disease, leishmaniasis is caused by a parasitic protozoans of leishmania genus and transmitted by female Phlebotomine sandflies. Depending on the body location where immotile form of the parasite namely amastigote is proliferated, three main clinical forms as cutaneous, muco-cutaneous and visceral leishmaniases are defined. While manifestation of cutaneous leishmaniasis is skin lesions on the exposed part of the body, enlarged lymph nodes, spleen or liver along with fever, fatigue and weight loss are the symptoms of visceral leishmaniasis. The most dangerous form is visceral leishmaniasis since it may end up with fatalities if patients are not treated. The purpose of this study was to investigate the difference between the protein expression profiles of leishmania isolates obtained from visceral and cutaneous leishmaniasis patients. To compare two sample groups to each other genetically, L.infantum was chosen since it causes both visceral and cutaneous leishmaniasis. Additionally, another sample group as cutaneous leishmaniasis caused by L.tropica was included to make the comparison both intra- and interspecies level. For protein profiling, both gel-based and gel-free proteomic approaches were carried out. In brief, a total of 15 samples, 5 from each group, were separated on pI 3-10 2D-PAGE gel. Additionally, 9 of those 15 samples, 3 from each group, were analyzed according to qualitative shotgun proteomics method and differential proteins were determined by drawing venn diagram.
2022-01-14 | PXD016333 | Pride