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ChIP-seq of Tbx20 tagged with GFP in E11.5 mouse hearts

ABSTRACT: ChIP-seq of Tbx20 tagged with GFP in E11.5 mouse hearts

PROVIDER: PRJEB11457 | ENA |

REPOSITORIES: ENA

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ChIP-seq of Tbx20 tagged with GFP in E11.5 mouse hearts

Project description:We performed ChIP-seq of Tbx20 tagged with GFP in mouse hearts to identify binding sites for this transcription factor. This data set is part of the study âEndocardial Tbx20 is essential for mesenchymal and myocardial cell movements required for cardiac septationâ.

2016-06-30 | E-MTAB-3967 | biostudies-arrayexpress

Genome-wide mapping of Tbx20 (GFP-tagged) in adult mouse whole heart

Project description:Tbx20 is a transcription factor important for heart development. To assess the role of Tbx20 in the adult heart, we sought to identify regulatory regions, gene targets and pathways regulated by this TF. To this end, we used chromatin immunoprecipitation (ChIP) of Tbx20 fused to GFP to locate binding sites in the genome of 8 weeks whole heart mouse tissue.

2011-11-21 | GSE29636 | GEO

Genome-wide mapping of Tbx20 (GFP-tagged) in adult mouse whole heart

2011-11-21 | E-GEOD-29636 | biostudies-arrayexpress

Gene expression analysis of control and Tbx20 mutant Tie2Cre lineage from E12.5 hearts

Project description:To investigate roles for Tbx20 in endocardium, we ablated Tbx20 utilizing Tie2Cre. Tie2Cre;Tbx20 mutants died at E14, exhibiting defects in multiple aspects of cardiac septation. Although endocardial cells lacking Tbx20 were able to undergo endothelial-to-mesenchymal transition, cushion mesenchymal cells lacking Tbx20 did not disperse normally. Non-cell autonomous roles of endocardial Tbx20 were also revealed, as evidenced by decreased myocardialization of outflow tract and failure of dorsal mesenchymal protrusion formation in mutants. To examine how ablation of Tbx20 in endocardial lineages affected gene expression, we performed global gene expression analysis on purified endocardial lineages. E12.5 hearts were dissociated, and Tie2Cre;RosatdTom lineage traced cells of controls and mutants were isolated by fluorescence activated cell sorting (FACS), after exclusion of blood cells (Ter119+, CD41+ and/or CD45+). Mutant endocardial lineages exhibited decreased expression of genes associated with extracellular matrix and cell migration. E12.5 hearts were dissociated, and Tie2Cre;RosatdTom lineage traced cells of controls and mutants were isolated by fluorescence activated cell sorting (FACS), after exclusion of blood cells (Ter119+, CD41+ and/or CD45+). FACS sorted Tie2Cre lineage from E12.5 hearts: Tie2Cre;Tbx20 +/loxP Control hearts versus Tie2Cre;Tbx20 loxP/- mutant hearts

2016-07-01 | E-GEOD-73858 | biostudies-arrayexpress

ATAC-seq on endothelial cells from E11.5 mouse hearts

Project description:ATAC-seq on endothelial cells from E11.5 mouse hearts

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Gene expression analysis of control and Tbx20 mutant Tie2Cre lineage from E12.5 hearts

2016-07-01 | GSE73858 | GEO

RNA-seq of wild-type and Tbx20 homozygous knockout E11.5 mouse cardiomyocytes to study Tbx20's roles in embryonic and adult mouse heart function

Project description:Tbx20 is a transcription factor known to play important roles in embryonic and adult mouse heart function. Our goal in this work was to better understand the function of this gene in embryonic (E11.5) mouse cardiomyocytes that form the developing chambers, expanding our knowledge of its role in heart development. To elucidate the role of Tbx20 in mouse cardiomyocytes, we generated conditional Tbx20 knockout and compared 4 samples with 4 samples of wild-type cardiomyocytes. We found evidence of regulation of cell cycle genes by Tbx20, which are involved in proliferation. In addition, Tbx20 seems to bind and regulate an enhancer of CoupTFII in the atrium, a gene involved in atrial development.

2018-06-14 | E-MTAB-5596 | biostudies-arrayexpress

IP-MS of GFP tagged MRV μ2 protein

Project description:To determine which cellular proteins interacts with the viral MRV protein μ2 and its mutant μ2-P208S, GFP-tagged constructions of μ2 and μ2-P208S in both C- and N- terminus were transfected in 293T cells alongside control GFP alone for 24h, and submitted to GFP pulldown before IP were prepared for MS analysis.

2022-06-09 | PXD029701 | Pride

ChIP-chip by array of GFP-tagged dFOXO expressed in Drosophila melanogaster adult gut and fat body

Project description:ChIP-chip by array of GFP-tagged dFOXO expressed in Drosophila melanogaster adult gut and fat body

2014-08-18 | E-MTAB-1021 | biostudies-arrayexpress

Bulk RNA sequencing of VE-Cadherin+GFP+, VE-Cadherin- GFP+ and VE-Cadherin+GFP- populations from E11.5 miR144/451+/GFP mouse yolk sac.

Project description:Bulk RNA sequencing of VE-Cadherin+GFP+, VE-Cadherin- GFP+ and VE-Cadherin+GFP- populations from E11.5 miR144/451+/GFP mouse yolk sac.

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