ABSTRACT: Ion Torrent based multi-gene sequencing as a reliable diagnostic assay for the detection of somatic mutations in tumors using minimal DNA amounts from FFPE material
Project description:Ion Torrent based multi-gene sequencing as a reliable diagnostic assay for the detection of somatic mutations in tumors using minimal DNA amounts from FFPE material - validation study
Project description:We show that semiconductor-based sequencing technology can be used to map mammalian replication domains, chromosomal units with similar DNA replication timing. Replicating DNA purified from mammalian cells was successfully sequenced by the Ion Torrent platform. The resultant replication domain map of mouse embryonic cells is comparable to those obtained by the conventional microarray-based method.
