Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Investigation of the transcriptional changes associated with phenotype switching in Streptococcus equi subspecies equi strain 1691.

ABSTRACT: Investigation of the transcriptional changes associated with phenotype switching in Streptococcus equi subspecies equi strain 1691.

PROVIDER: PRJEB12198 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Similar Datasets

Investigation of the transcriptional changes associated with phenotype switching in Streptococcus equi subspecies equi strain 1691.

Project description:Streptococcus equi subspecies equi, strain 1691 grown on COBA streptococcal selective agar shows classical mucoid colony morphology in addition to a reduced capsule phenotype. This project aimed to identify changes in the transcriptional profile between the two morphologies.

2016-12-15 | E-MTAB-4147 | biostudies-arrayexpress

Comprehensive proteomics for Discovery vaccine candidates against Streptococcus equi Infections

Project description:Streptococcus equi subspecies equi (S. equi) is a major pathogen which cause strangles, a highly contagious respiratory infection, in horses and other equines. In this study, we purified the extracellular vesicles (EVs) of S. equi ATCC 39506 and evaluated them as vaccine candidates against S. equi infections in mice. Through immunization in an animal model and immunoprecipitation-mass spectrometry, we evaluated EV as vaccine candidates against S. equi infections and identified novel immunogenic proteins.

2021-08-06 | PXD025527 | Pride

Comprehensive proteomics for Discovery vaccine candidates against Streptococcus equi Infections

Project description:Streptococcus equi subspecies equi (S. equi) is a major pathogen which cause strangles, a highly contagious respiratory infection, in horses and other equines.In this study, we discovered potential vaccine candidates using comprehensive proteomics and reverse vaccinology. As the initial step, we divided proteome of S. equi ATCC 39506 into whole cell lysate, secretory proteome, membrane proteome and extracellular vesicle and then, comparative proteomic analysis was performed to characterize the functional features of the proteome. Especially, extracellular vesicle of S. equi was evaluated at the first time. Total 114 potential vaccine candidates (PVCs) were selected using reverse vaccinology and knowledge based annotations. Comprehensive proteomic analysis confirmed that 60 PVCs were identified in S. equi ATCC 39506. Particularly, 32 PVCs were enriched in the EV proteome, suggesting that this cellular fraction may serve as vaccine.

2021-08-06 | PXD025152 | Pride

1691 phenotype

Project description:Genome sequence of two phenotypes from Streptococcus equi strain 1691.

| PRJEB9200 | ENA

RNA sequencing of Streptococcus equi subsp. equi collected from carrier and acute cases

Project description:Streptococcus equi subsp. equi (SEE) is a host-restricted bacterium that causes the common infectious upper respiratory disease known as strangles in horses. Perpetuation of SEE infection appears attributable to inapparent carrier horses because it does not persist long-term in the environment, infect other host mammals or vectors, and result in short-lived immunity. Whether pathogen factors enable SEE to remain in horses without causing clinical signs remains poorly understood. Thus, our objective was to use next-generation sequencing technologies to characterize the transcriptome of isolates of SEE from horses with acute clinical strangles and inapparent carrier horses to assess pathogen-associated changes that might reflect adaptions of SEE to the host contributing to inapparent carriage. RNA sequencing of SEE isolates from Pennsylvania demonstrated no genes that were differentially expressed between acute clinical and inapparent carrier isolates of SEE.

2021-05-28 | GSE167862 | GEO

Streptococcus equi subsp. equi

Project description:Streptococcus equi subsp. equi Genome sequencing and assembly

| PRJNA214018 | ENA

Streptococcus equi subsp. equi

Project description:Streptococcus equi subsp. equi Genome sequencing and assembly

| PRJNA805196 | ENA

2013-05-31 | GSE45004 | GEO

Project description:The objectives of this study were 2-fold: 1) to compare the expression profiles at specific ages of blood leukocytes from foals stimulated with virulent R. equi with those of unstimulated leukocytes; and, 2) to characterize the age-related changes in the gene expression profile associated with blood leukocytes in response to stimulation with virulent R. equi. Peripheral blood leukocytes were obtained from 6 foals within 24 hours (h) of birth (day 1) and 2, 4, and 8 weeks after birth. The samples were split, such that half were stimulated with live virulent R. equi, and the other half served as unstimulated control. For one hyb design: we hybridized 6 samples stimulated and 6 unstimulated at each time point (30 mL of blood was treated with live virulent R. equi (Strain ATCC 33701) and the remaining 30 mL of blood with PBS ). For time course: 6 samples from day1 stimulated hybridized to week2 stimulated; day1 stimulated hyb to week 4 stimulated and day1 stimulated hyb to week8 stimulated. RNA was extracted and the generated cDNA was labeled with fluorescent dyes for microarray hybridizations using an equine microarray.

2013-05-31 | E-GEOD-45004 | biostudies-arrayexpress

Streptococcus equi subsp. equi 4047

Project description:Streptococcus equi subsp. equi 4047 Raw sequence reads

| PRJNA379394 | ENA