Project description:Development of an updated genome-scale metabolic model of Clostridium thermocellum and its application for integration of multi-omics datasets
Project description:Clostridium botulinum ATCC 3502 was grown in continuous culture at 39°C, subjected to heat shock at 45°C, and then continuously grown at 45°C. The goal was to determine the impact of stressful temperature to the whole genome expression profile and identify stress adaptation mechanisms.
Project description:Genomic DNA of 61 strains of proteolytic Clostridium botulinum or Clostridium sporogenes was subjected to analysis by DNA microarray.
Project description:Clostridium phytofermentans was recently isolated from forest soil and is distinguished by its capacity to directly ferment plant cell wall polysaccharides into ethanol as the primary product, suggesting that it possesses unusual catabolic pathways. The objective of the present study was to understand the molecular mechanisms of biomass conversion to ethanol in a single organism, Clostridium phytofermentans, by analyzing its complete genome and transcriptome during growth on plant carbohydrates. The saccharolytic versatility of C. phytofermentans is reflected in a diversity of genes encoding ATP-binding cassette sugar transporters and glycoside hydrolases, many of which may have been acquired through horizontal gene transfer. These genes are frequently organized as operons that may be controlled individually by the many transcriptional regulators identified in the genome. Preferential ethanol production may be due to high levels of expression of multiple ethanol dehydrogenases and additional pathways maximizing ethanol yield. The genome also encodes three different proteinaceous bacterial microcompartments with the capacity to compartmentalize pathways that divert fermentation intermediates to various products. These characteristics make C. phytofermentans an attractive resource for improving the efficiency and speed of biomass conversion to biofuels.
Project description:This SuperSeries is composed of the following subset Series: GSE12358: Clostridium beijerinckii NCIMB 8052 wild-type fermentation time course GSE12359: Clostridium beijerinckii BA101 mutant fermentation time course Refer to individual Series
Project description:Clostridium acetobutylicum is a Gram-positive, endospore-forming bacterium that is considered as a strict anaerobe. It ferments sugars to the organic acids acetate and butyrate or shifts to formation of the solvents - ethanol, butanol and acetone. In most bacteria the major regulator of iron homeostasis is Fur (ferric uptake regulator). Analysis of the genome of Clostridium acetobutylicum has revealed three genes encoding Fur-like proteins. The amino acid sequece of one of them showed 70% similarity to the Fur protein of the closely related Bacillus subtilis.<br>Thus, to gain insight into the role of Fur and the mechanisms for maintenance of iron homeostasis in this strict anaerobic organism, we determined its transcriptional profile in response to iron limitation and inactivation of fur.
Project description:Clostridium botulinum ATCC 3502 was grown in continuous culture at 39°C, subjected to heat shock at 45°C, and then continuously grown at 45°C. The goal was to determine the impact of stressful temperature to the whole genome expression profile and identify stress adaptation mechanisms. Time series (control,0min,10min,1h,18h,42h, adapt) with two biological replicates and two technical replicates in dye-swaps. Common reference hybridization design.
