Project description:In frame of the IBSC The Genome Analysis Centre (TGAC) sequenced BACs, that constitute the MTP of barley chromosome 2H using MatePair Pool Sequencing.
Project description:In frame of the IBSC The Genome Analysis Centre (TGAC) sequenced BACs, that constitute the MTP of barley chromosome 2H using Illumina 2000 chemistry.
Project description:In frame of the IBSC The Genome Analysis Centre (TGAC) sequenced BACs, that constitute the MTP of barley using Illumina 2000 chemistry. The BACs presented here could not be assigned to any barley chromosome yet and were therefore assigned as 0H.
Project description:BAC pool DNA hybridisation of barley to 44k Agilent microarrays. We have used two-channel Agilent expression microarrays to address thousands of gene sequences to individual BAC clones and contigs that form part of an emerging physical map of the large and unsequenced 5300 Mbp barley genome. By using two-colour processing, each array allows simultaneous co-hybridization of two independent BAC pools (SP), for which the data is analysed separately. As a general approach the method represents a cost-effective, highly parallel alternative to traditional gene-to-BAC addressing methods. By coupling the BAC address-data with gene-based genetic maps we were able to anchor thousands of BACs to the barley genetic map.
Project description:In frame of the IBSC The Genome Analysis Centre (TGAC) sequenced BACs, that constitute the MTP of barley using MatePair Pool Sequencing. The BAC Pools presented here could not be assigned to any barley chromosome and were therefore assinged 0H.
Project description:Barley (Hordeum vulgare L.) is amongst the oldest domesticated crop plants and remains one of the world's most important crop species. It is diploid with a haploid genome of 5.1 gigabases (Gb), twice the size of those of human and maize, and closely related to the most widely grown crop, hexaploid wheat. To meet global demand for food, feed and fibre, it is commonly agreed that reference genome sequences of our crop plants are urgently required to enable genome-assisted crop improvement. As part of the The International Barley Genome Sequencing Consortium (IBSC) we present here raw data obtained from Illumina (GAII) sequencing of RNA samples from nine different barley cultivars for the purpose of Single Nucleotide Polymorphism (SNP) discovery.
