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Microbial analysis of TMAH-degrading consortia

ABSTRACT: Barcoded High-Throughput Sequencing Analysis Revealed Direct and Indirect Methanogenesis of Tetramethylammonium Hydroxide in Ambient Methanogenic Reactors

PROVIDER: PRJEB14151 | ENA |

REPOSITORIES: ENA

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Analysis of TMAH-degrading microbial community

Project description:High-throughput Barcoded Sequencing Analysis Revealed Direct and Indirect Methanogenesis of Tetramethylammonium Hydroxide (TMAH) in Ambient Methanogenic Reactors

| PRJEB13976 | ENA

Genomic hybridization of Methanobacteria to identify the diversity in strains on manure and olive mill waste.

Project description:Population dynamics of methanogenic genera was investigated in pilot anaerobic digesters. Cattle manure and two-phase olive mill wastes were codigested at a 3:1 ratio in two reactors operated at 37 ï¾°C and 55 ï¾°C. Other two reactors were run with either residue at 37 ï¾°C. Sludge DNA extracted from samples taken from all four reactors on days 4, 14 and 28 of digestion was used for hybridisation with the AnaeroChip, an oligonucleotide microarray targeting those groups of methanogenic archaea that are commonly found under mesophilic and thermophilic conditions (Franke-Whittle et al. 2009, in press, doi:10.1016/j.mimet.2009.09.017).

2010-11-10 | E-MEXP-2453 | biostudies-arrayexpress

archaeon R1 strain:methanogenic propionate degrading microbial consortia

Project description:methanogenic propionate degrading microbial consortia

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Dispensable Methanogenic Energy Metabolism in a Methanogenic Archaeon

Project description:Biogenic methane formation, methanogenesis, a key process in the global carbon cycle is the only energy metabolism known to sustain growth of the microorganisms employing it, the methanogenic archaea. All known methanogenic pathways converge at the methane-liberating step where also the terminal electron acceptor of methanogenic respiration, the heterodisulfide of coenzyme M and coenzyme B is formed. Carbon monoxide (CO) utilization of Methanosarcina acetivorans is unique in that the organism can shift from methanogenesis towards acetogenesis. Here, we show that M. acetivorans can dispense of methanogenesis for energy conservation completely. By disrupting the methanogenic pathway through targeted mutagenesis, followed by adaptive evolution, a strain capable of sustained growth by CO-dependent acetogenesis was created. Still, a minute flux through the methane-liberating reaction remained essential, which was attributed to the involvement of the heterodisulfide in at least one essential anabolic reaction. Genomic and proteomic analysis showed that substantial metabolic rewiring had occurred in the strain. Most notably, heterodisulfide reductase, the terminal respiratory oxidoreductase was eliminated to funnel the heterodisulfide towards anabolism. These results suggest that the metabolic flexibility of “methanogenic” archaea is much greater than anticipated and open avenues for probing the mechanism of energetic coupling and the crosstalk between catabolism and anabolism.

2021-10-05 | PXD022016 | Pride

M.ruminantium B.proteoclasticus coculture vs monocultures

Project description:This study was undertaken with two major goals in mind: i) To investigate the potential for interspecies hydrogen transfer between a hemicellulytic rumen bacterium (B. proteoclasticus) and a methanogenic achaea (M. ruminantium) Microscopic examination had previously shown B. proteoclasticus in co-culture with M. ruminantium rapidly formed cell to cell co-aggregates. ii) To examine the expression of genes involved in methanogenesis under more rumen-like conditions.

2010-01-28 | GSE18716 | GEO

New mode of methanogenesis observed in the coal degrading methanogen Methermicoccus shengliensis

Project description:Methane-generating Archaea drive the final step in anaerobic organic compound mineralization and dictate the carbon flow of Earth’s diverse anaerobic ecosystems. Although such Archaea were presumed to be restricted to life on simple compounds like H2, acetate or methanol, an archaeon, Methermicoccus shengliensis, was recently found to convert methoxylated aromatic compounds to methane. Methoxylated aromatic compounds as component of lignin and coal are present in most subsurface sediments. Despite the significance and novelty of this outstanding archaeon its metabolism has not yet been explored. In this study, transcriptomics and proteomics reveal that M. shengliensis uses a demethoxylation system that is more related to that from acetogenic bacteria than to the methyl transferase system used for methylotrophic methanogenesis. It activates methoxy-groups using tetrahydromethanopterin as the carrier, a mechanism distinct from conventional methanogenic methyl-transfer systems dependent on Coenzyme M.

2021-06-20 | PXD018934 | Pride

ACE-degrading consortia

Project description:Acesulfame aerobic biodegradation by enriched consortia and Chelatococcus spp.: kinetics, transformation products and genomic characterization

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Oleate-degrading enrichment cultures

Project description:Methanogenic and non-methanogenic oleate-degrading enrichment cultures

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Next Generation Metatranscriptome Sequencing of an Anaerobic Hexadecane-Degrading Microbial Consortia

Project description:Purpose: Identification of transcriptionally active genes in the unculturable community constituent, Smithella, during hexadecane degradation; Differential gene expression analysis of hexadecane-relevant genes acoss three different conditions; Extension of metatranscriptomic datasets to other community constituents to identify interspecies relationships. mRNA profiles were generated for this community across three different conditions (hexadecane-, butyric acid-, caprylic acid-degrading conditions) using a modified version of Nextera and sequenced using Illumina's Miseq platform.

2013-10-29 | E-GEOD-49830 | biostudies-arrayexpress

Anaerobic ACE-degrading consortia

Project description:Anoxic acesulfame biodegradation coupled to nitrate reduction

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