Project description:We performed RNA-seq experiments Mus caroli and Mus pahari to aid in annotating their genomes. RNA was extracted from multiple tissues: brain, liver, heart and kidney. Complementary ChIP-seq data in this study have also been deposited in ArrayExpress, under accesison number E-MTAB-5769 ( https://www.ebi.ac.uk/arrayexpress/experiments/E-MTAB-5769/ )
Project description:We explored the relationship between the evolutionary dynamics of CTCF binding and the functional stability of higher order genome structures, by performing ChIP-seq experiments in closely related Mus species or strains and intersecting with Hi-C-derived topologically associating domains (TADs) and expression data. To complement our data analyses, we generated RNA-seq libraries from two Mus caroli adult male individuals.
Project description:We explored the microevolutionary trends of CTCF binding evolution by preforming ChIP-seq experiments in five closely related Mus strains, subspecies and species: Mus musculus domesticus, Mus musculus castaneus, Mus spretus, Mus caroli and Mus pahari. All experiments were performed in adult male liver samples in 3 biological replicates and with an input control set. Complementary RNA-seq data from this same study have been deposited in ArrayExpress under accession numebr E-MTAB-5768 ( https://www.ebi.ac.uk/arrayexpress/experiments/E-MTAB-5768 ).
