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Oryza sativa ssp. indica Illumina lwhole-genome bisulfite sequencing used for coverage comparison next to the method plant-RRBS

ABSTRACT: Oryza sativa ssp. indica Illumina lwhole-genome bisulfite sequencing used for coverage comparison next to the method plant-RRBS

PROVIDER: PRJEB15109 | ENA |

REPOSITORIES: ENA

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Oryza sativa ssp. indica Illumina lwhole-genome bisulfite sequencing used for coverage comparison next to the method plant-RRBS

Project description:The contained data consist of Illumina HiSeq reads generated genomic DNA of Oryza sativa ssp. indica used for comparative coverage aspects with plant-RRBS methylome profiling by bioinformatics analyses. The inbred control line and a derived epiline LR2 of the 4th selfing were analysed using whole-genome bisulfite sequencing.

2017-12-14 | E-MTAB-5002 | biostudies-arrayexpress

Oryza sativa ssp. indica Illumina libraries of the method plant-RRBS used for methylome profiling

Project description:The contained data consist of Illumina HiSeq 2500 reads generated from restriction endonuclease digested genomic DNA of Oryza sativa ssp. indica used as proof of concept for plant-RRBS methylome profiling by bioinformatics analyses. Five biological repeats for an inbred control line and a derived epiline of the 4th generation where analysed using two restriction endonuclease combination (MspI-DpnII or MspI-ApekI).

2017-12-14 | E-MTAB-4626 | biostudies-arrayexpress

Oryza sativa ssp. indica Illumina libraries of the method plant-RRBS used for methylome profiling

Project description:Oryza sativa ssp. indica Illumina libraries of the method plant-RRBS used for methylome profiling

| PRJEB13320 | ENA

High concordance between Illumina HiSeq2500 and NextSeq500 for reduced representation bisulfite sequencing (RRBS).

Project description: Not available

| S-EPMC5065634 | biostudies-literature

Plant-RRBS, a bisulfite and next-generation sequencing-based methylome profiling method enriching for coverage of cytosine positions.

Project description: Not available

| S-EPMC5501559 | biostudies-literature

High concordance between Illumina HiSeq2500 and NextSeq500 for Reduced Representation Bisulfite Sequencing (RRBS)

Project description:Reduced representation bisulfite sequencing (RRBS) provides an efficient method for measuring DNA methylation at single base resolution in regions of high CpG density. This technique has been extensively tested on the HiSeq2500, which uses a 4-colour detection method, however it is unclear if the method will also work on the NextSeq500 platform, which employs a 2-colour detection system. We created an RRBS library and sequenced it on both the HiSeq2500 and NextSeq500, and found no significant difference in the base composition of reads derived from either machine. Moreover, the methylation calls made from the data of each instrument were highly concordant, with methylation patterns across the genome appearing as expected. Therefore, RRBS can be sequenced on the Nextseq500 with comparable quality to that of the HiSeq2500.

2017-02-09 | GSE87097 | GEO

Illumina whole-genome bisulfite sequencing of Oryza sativa ssp. indica inbred control and epilines

Project description:The contained data consist of Illumina HiSeq reads generated genomic DNA of Oryza sativa ssp. indica used for WGBS analysis. The inbred control line and derived epilines LR1, LR2 and LR3 of the 4th selfing were analysed using whole-genome bisulfite sequencing. Control and LR2 lines can be found in ArrayExpress E-MTAB-5002 submission, LR1 and LR3 are part of this submission

2020-06-21 | E-MTAB-6981 | biostudies-arrayexpress

Q-RRBS: a quantitative reduced representation bisulfite sequencing method for single-cell methylome analyses.

Project description: Not available

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Rapid and affordable genome-wide bisulfite DNA sequencing by XmaI-RRBS

Project description:We describe XmaI-RRBS method for rapid and affordable genome-wide DNA methylation analysis, with library preparation taking only four days and sequencing possible within four hours. Small sizes of the XmaI-RRBS libraries allow their multiplexing and sequencing on the benchtop high-throughput machines. Described here is the first RRBS protocol validated for the Ion Torrent Personal Genome Machine. DNA from MCF7 cell line and 6 normal breast samples (total 7 samples) were subjected to reduced representation bisulfite sequencing analysis (XmaI-RRBS) by using Ion Torrent platform.

2015-11-01 | E-GEOD-74126 | biostudies-arrayexpress

RRBS (Reduced Representation Bisulfite Sequencing) of HL60

Project description:We exploited the methylation genome-scale screening RRBS to correlate the RNA species physically associated with DNMT1 and proximal to the annotated genes to the methylation status of the corresponding loci. Out of 15275 non ambiguous gene loci identified by DNMT1 RIP-Seq, 9436 loci were covered by RRBS. These 9436 loci were clustered according to the fold of specific DNMT1 library peaks enrichment (defined as the ratio of the sum of the area under the curve of specific DNMT1 library peaks covering the gene loci). Genes were then stratified by the expression profile ultimately leading to the epitranscriptome map, a comprehensive map cross-referencing DNMT1-interacting transcripts to (i) DNA methylation and (ii) gene expression profile. Relationship between DNMT1-RNA interactions, DNA methylation and gene expression

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