Project description:We measured steady-state transcript levels in 1) wild-type E. litoralis DSM 8509, 2) a nepR-ecfG deletion strain (ΔnepR-ecfG), 3) a phyR deletion strain (ΔphyR), 4) a lovK-lovR deletion strain (ΔlovKR), and 5) a gsrK-gsrP deletion strain (ΔgsrKΔgsrP) cultivated in constant white light or in constant dark conditions.
Project description:The development of proteins hyper-production platform in thermophilic fungus Myceliophthora thermophila is essential for both molecular basis understanding and industrial process. Herein the glucoamylase hyper-production strain MtGM12 was generated from our previously strain MtYM6 via genetically engineering. Transcriptional profiling analyses revealed that the amylolytic gene expression levels were significantly up-regulated in the MtGM12 than in MtWT.