Project description:We used 10X Genomics Chromium system to profile single cells from a pool of Wild-type Ciona robusta embyros at 12 hours post fertilization (hpf)
Project description:This study was carried out to compare the changes in gene expression in whole larvae 120 hours post fertilization zebrafish larvae in response to whole body mutation of the arsenic methylation gene (as3mt) - 8 bp deletion in exon 3 with and without 6-120 hpf exposure to 1 mM sodium arsenite (iAs).
Project description:This dataset was acquired to identify the gene expression profile of developing retinal cell types at 42 hours post fertilization (hpf): retinal ganglion cells, photoreceptors, inhibitory neurons (amacrine cells and horizontal cells) and progenitor cells.
Project description:The goal of this experiment was to evaluate the expression profile of cardiomyocytes freshly isolated from zebrafish embryos. Here we report mRNA abundance measurements for zebrafish cardiomyocytes at 20 hours post fertilization (hpf). We found that cardiomyocytes isolated from zebrafish embyros are enriched for orthologues of genes abundant in mammalian iPS-derived cardiomyocytes and other in vitro models of cardiomyocytes. It was also enriched with genes required for cardiac development in zebrafish and mammalian models. Using a rapid-throughput CRISPR-Cas9 based G0 screening pipeline, we tested 50 abundant transcription factors derived from this profile, rediscoved transcription factors known to be required for cardiac development, and found a novel role for zbtb16a in cardiac development.
Project description:Exposure to ethanol causes liver injury and activates stress response pathways. Here we compared gene expression in the zebrafish larval liver between larvae that were untreated and those exposed to 175 mM and 350 mM ethanol from 96-120 hours post fertilization (hpf).
Project description:We report single cell gene expression data for caudal tail tissue cells collected from embryos at 72 hours post fertilization (hpf).
Project description:Transcriptional profiling of zebrafish embryo comparing wild type untreated embryos with embryos injected with morpholino of zf-grna. This assay is used for determination of expression profiling of trunk muscle at 16, 24, 48, 72 hpf under GRN-A deficiency.
Project description:We report single cell gene expression data for FACS-isolated zebrafish kdrl(flk1):GFP positive cells collected from whole embryos at 72 hours post fertilization (hpf).