Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

OPLAH prevents accumulation of cardiotoxic 5-oxoproline after ischemic injury and improves cardiac function

ABSTRACT: OPLAH prevents accumulation of cardiotoxic 5-oxoproline after ischemic injury and improves cardiac function

PROVIDER: PRJEB19249 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Similar Datasets

OPLAH prevents accumulation of cardiotoxic 5-oxoproline after ischemic injury and improves cardiac function

Project description:In response to heart failure (HF), the heart reacts by repressing adult genes and expressing fetal genes, thereby returning to a more fetal-like gene profile. To identify genes involved in this process, we carried out transcriptional analysis on murine hearts at different stages of development and adult mice with HF. Our screen identified 5-oxoprolinase (OPLAH), a member of the -Glutamyl cycle, that functions by scavenging 5-oxoproline. OPLAH depletion occurred as a result of cardiac injury, leading to elevated 5-oxoproline and oxidative stress, whereas OPLAH overexpression improved cardiac function after ischemic injury. In HF patients we observed elevated plasma 5-oxoproline levels, which were associated with a worse clinical outcome. Understanding and modulating fetal-like genes in the failing heart may lead to potential novel diagnostic, prognostic and therapeutic options in HF.

2017-11-08 | E-MTAB-5449 | biostudies-arrayexpress

NLRP3-inflammasome suppression improves longevity and prevents cardiac aging in mice

Project description:NLRP3-inflammasome suppression improves longevity and prevents cardiac aging in mice

| PRJNA512138 | ENA

Rejuvenation improves the flavonoid accumulation in Ginkgo biloba leaves

Project description:Rejuvenation improves the flavonoid accumulation in Ginkgo biloba leaves

| PRJNA629818 | ENA

ZFP281-BRCA2 prevents R-loop accumulation during DNA replication

Project description:ZFP281-BRCA2 prevents R-loop accumulation during DNA replication

| PRJNA524627 | ENA

Protein affects gene expression and prevents lipid accumulation in the liver in mice

Project description:Obesity and associated increased prevalence of non-alcoholic fatty liver (NAFLD) disease is suggested to be positively modulated by a high protein (HP) diet in humans and rodents. The aim was to detect mechanisms by which a HP diet prevents hepatic lipid accumulation by means of transcriptomics. To study the acute and long term effect of a high protein ingestion on hepatic lipid accumulation under both low and high fat (HF) conditions, mice were fed combinations of high (35%) or low (10%) fat and high (50%) or normal (15%) protein diets for 1 or 12 weeks. Body composition, liver fat, VLDL production rate and gene expression were investigated. Differences in metabolic processes and functions in the liver were identified using gene set enrichment analysis on microarray data. Mice fed the HP diets developed less adiposity and decreased hepatic lipid accumulation due a combination of induced processes mainly involved in protein catabolism such as transamination, TCA cycle and oxidative phosphorylation. Feeding a HP diet can successfully prevent the development of NAFLD by using ingested energy for oxidation instead of storage. Wild type mice were fed combinations of high (35%) or low (10%) fat and high (50%) or normal (15%) protein diets for 1 or 12 weeks. After the diet intervention period, the animals were killed and liver tissue was removed. Total RNA was isolated, pooled and subjected to gene expression profiling.

2012-11-21 | E-GEOD-37897 | biostudies-arrayexpress

Mannose alters gut microbiome, prevents diet induced obesity and improves host metabolism

Project description:Mannose alters gut microbiome, prevents diet induced obesity and improves host metabolism

| PRJNA434573 | ENA

Linker histone H1 prevents R-loop accumulation and genome instability in heterochromatin

Project description:Linker histone H1 prevents R-loop accumulation and genome instability in heterochromatin

| PRJNA387003 | ENA

ZEB2 protects the heart from ischemic damage

Project description:infarct size and subsequent deterioration in function. The identification of factors that enhance cardiac repair by the restoration of the vascular network is, therefore, of great significance. Here, we show that the transcription factor Zinc finger E-box-binding homeobox 2 (ZEB2) is increased in stressed cardiomyocytes and induces a cardioprotective cross-talk between cardiomyocytes and endothelial cells to enhance angiogenesis after ischemia. Single-cell sequencing indicates ZEB2 to be enriched in injured cardiomyocytes. Cardiomyocyte-specific deletion of ZEB2 results in impaired cardiac contractility and infarct healing post-myocardial infarction (post-MI), while cardiomyocyte-specific ZEB2 overexpression improves cardiomyocyte survival and cardiac function. We identified Thymosin 4 (TMSB4) and Prothymosin (PTMA) as main paracrine factors released from cardiomyocytes to stimulate angiogenesis by enhancing endothelial cell migration, and whose regulation is validated in our in vivo models. Therapeutic delivery of ZEB2 to cardiomyocytes in the infarcted heart induces the expression of TMSB4 and PTMA, which enhances angiogenesis and prevents cardiac dysfunction. These findings reveal ZEB2 as a beneficial factor during ischemic injury, which may hold promise for the identification of new therapies.

2021-03-23 | PXD022212 | Pride

Neovascularization of ischemic myocardium by newly isolated tannins prevents cardiomyocyte apoptosis and improves cardiac function.

Project description: Not available

| S-EPMC1829195 | biostudies-literature

Transcriptomic profiling of cardiac biopsies from mice subjected to ischemic preconditioning

Project description:Microarray profiling of cardiac biopsies from the area at at risk and remote myocardium of mice subjected to ischemic preconditioning. Mice were subjected to ischemic preconditioning (IPC) through reversible ligation of the left coronary artery or a sham procedure. The procedure consisted of 4 minutes of ischemia followed by 4 minutes of reperfusion, repeated 4 times and then followed by a 30 minute reperfusion period. Biopsies were taken from the area at risk (AAR) and remote myocardium (RM) from two IPC mice and two sham control mice.

2016-06-24 | E-GEOD-83659 | biostudies-arrayexpress

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data