Project description:RBP10 was induced for 24 or 48 hours in procyclic cells derived from EATRO1125; this primes the procyclic cells to differentiate to bloodstream form. RNA-seq was performed to quantify mRNAs changes during the differentiation triggered by RBP10 overexpression.
Project description:A direct comparison of RNAi in vitro with RNAi in vivo is being performed using RNA interference (RNAi) target sequencing (RIT-Seq) of Trypanosoma brucei to identify all genes specifically required for growth in vivo (the infectome). Assembly of the bloodstream-form T. brucei RNAi library and the RNAi target sequencing (RIT-seq) approach in African trypanosomes were reported previously in Alsford, S. et al. High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome. Genome Res 21, 915-924, 264 doi:gr.115089.110 [pii] 265 10.1101/gr.115089.110 (2011) and Alsford,S et al. High-throughput decoding of antitrypanosomal drug efficacy and resistance. Nature 482, 232236 doi:10.1038/nature10771 (2012). This data is part of a pre-publication release. For information on the proper use of pre-publication data shared by the Wellcome Trust Sanger Institute (including details of any publication moratoria), please see http://www.sanger.ac.uk/datasharing/
Project description:In this study we investigate the function of RBP10, and present evidence that the presence of RBP10 is sufficient to induce the expression of mRNAs involved in bloodstream-form energy metabolism.
Project description:In this study we investigate the function of RBP10, and present evidence that the presence of RBP10 is sufficient to induce the expression of mRNAs involved in bloodstream-form energy metabolism. for BS RBP10 RNAi Samples: total of 6 slides, including 3 biological replicates and dyeswap for RBP10 overexpression Samples: total of 5 slides, including 3 biological replicates and dyeswap