Ontology highlight
ABSTRACT:
PROVIDER: PRJEB23136 | ENA |
REPOSITORIES: ENA
Similar Datasets
Project description:Transcriptome analysis of the oriental fruit fly Bactrocera dorsalis early embryos
| PRJNA485742 | ENA
Project description:STING is a protein that plays important role in innate immune response. However, it also has functions not related to immunity. We studied role of STING in fruit fly Drosophila melanogaster. We used microarray to detect gene expression changes in dSTING knockout fruit flies. We studied role of STING in fruit fly Drosophila melanogaster. To detect gene expression changes in dSTING-knockout flies microarray assay was used.
2021-02-22 | GSE167164 | GEO
Project description:Purpose: the goals of this study are to compare fruit of two clitivars oriental melon transcriptome profiling (RNA-seq) at different stages to explore carotenoid potentail carotenoid accumulation mechanism Methods:The transcriptome sequence of two cultivars oriental melon fruits at different stages were generated by deep sequencing with three repeats using Illumina. The sequence reads that passed filters were mapped to melon genome (http://cucurbitgenomics.org/organism/18) using HISAT2 software. The differently expressed genes were identify by |log2(FoldChange)| > 0 & padj <= 0.05, and qRT–PCR validation was performed using SYBR Green assays Result:Using an optimized data analysis workflow, we mapped about 40 million sequence reads per sample to the melon genome. The differentially expressed genes were functionally classified by GO and KEGG enrichment. We focused on carotenoid metabolism related gene and validated using qRT-PCR. The results showed RNA-seq and qRT-PCR were highly correlated. Conclusion: Our study provided transcriptome sequence of oriental melon fruits at different stages in two cultivars. The optimized data analysis workflows reported here should provide comparative framework of expression profiles. Our transcriptome characterization contribute to analyze gene functions and metabolic process of oriental melon.
2022-01-13 | GSE193412 | GEO
Project description:The distribtion of H3K18ac in the fruit fly genome was analyzed in wing imaginal discs treated for 2 h with etomoxir and compared to control discs.
2024-04-06 | GSE207486 | GEO
Project description:Intestinal responses of the oriental fruit fly Bactrocera dorsalis after ingestion of an entomopathogenic bacterium strain
| PRJNA543205 | ENA
Project description:enrichment of glycoproteins in fruit fly (Drosophila) using GNA, RSA and Nictaba lectin
2011-03-18 | PRD000822 | Pride
Project description:We performed a genome-wide ChIP analysis to find the set of target genes regulated by Cbt in Drosophila wing imaginal discs. We reported multiple transcriptional regulators and genes involved in the developlment, growth and patterning of the fruit fly.
2015-02-20 | GSE40958 | GEO
Project description:A very common co-translational modification in multicellular organisms is the addition of carbohydrate structures called glycans to the peptide backbone. This important protein modification is called protein glycosylation. In this report, the diversity in protein glycosylation between insect species covering a wide phylogenetic distance was analyzed. Based on previous data of the glycan profile from the fruit fly (Drosophila melanogaster), the oligomannose N- glycan binding snowdrop lectin (Galanthus nivalis agglutinin, GNA) was selected for affinity chromatography of glycoproteins. The insect species that were used in this study were the flour beetle (Tribolium castaneum), the silkworm (Bombyx mori), the honey bee (Apis mellifera), the fruit fly (D. melanogaster) and the pea aphid (Acyrthosiphon pisum). To identify and characterize the purified glycoproteins, LC-MS/MS analysis was performed. For all insect species, it was demonstrated that glycoproteins are involved in a broad range of biological processes and molecular functions. However, major differences between insect species were observed for particular glycosylated proteins. This finding was reflected by differences in the relative abundance of particular proteins for certain biological processes or molecular functions. Besides the abundant presence of oligomannose N-glycans on insect glycoproteins, we also showed that O-mannosylation events occur more frequently than currently anticipated.
2011-03-18 | PRD000276 | Pride
Project description:We used SOLiD technology to deep sequence libraries prepared from 18-35 nt PAGE purfied small RNAs derived from female Drosophila. The objective of our study was to compare the impact of R2D2 or loquacious mutantions on different small RNA populations in the fruit fly.
2009-11-04 | GSE18871 | GEO