Bovine Rumen Microbiome
Ontology highlight
ABSTRACT:
PROVIDER: PRJEB23561 | ENA |
REPOSITORIES: ENA
Similar Datasets
Project description:The goal of this study was to characterize a single-cell clone derived from bovine rumen epithelium. Analyses including RNA-seq demonstrated that this clone was derived from a rumen epithelial cell. This clone is named BREC1 in the manuscript.
2021-10-27 | GSE181659 | GEO
Project description:We evaluated the effects of pure glyphosate and the formulations Durano TF and Roundup® LB plus in different concentrations on rumen microbiota bioreactor model using the Rumen Simulation Technique (RUSITEC). Application of the compounds in three concentrations (0.1 mg/L, 1.0 mg/L or 10 mg/L, n = 4 each).
2022-05-08 | PXD032166 | Pride
Project description:Bovine rumen metagenome under glyphosate influence
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Project description:GIZ7 Bovine rumen bacteria Raw sequence reads
| PRJNA454463 | ENA
Project description:Metatranscriptome sequencing reveals insights into the gene expression of the bovine rumen wall microbial community during subacute ruminal acidosis
| PRJEB22186 | ENA
Project description:The time-resolved impact of monensin on the active rumen microbiome in a rumen-simulating technique (Rusitec) was studied with metaproteomic and metabolomic approaches. Upon monensin treatment, decreased catabolism linked to fiber degradation was observed by the reduced abundance of proteins assigned to fibrolytic bacteria and glycoside hydrolases, sugar transporters and carbohydrate metabolism. Reduced amounts of ammonium as well as branched-chain fatty acids pointed towards a decreased proteolytic activity. The family Prevotellaceae exhibited increased resilience in the presence of monensin, with a switch of metabolism from acetate to succinate production. Prevotella species harbor a membrane bound electron transfer complex, which drives the reduction of fumarate to succinate, the substrate for propionate production in the rumen habitat. Besides the increased succinate production, a concomitant depletion of methane concentration was observed upon monensin exposure. Our study demonstrates that Prevotella sp. shifts its metabolism successfully in response to monensin exposure and Prevotellaceae represents the key bacterial family stabilizing the rumen microbiota during exposure to monensin.
2022-12-11 | PXD034099 | Pride
Project description:The rumen microbiota of finishing beef cattle divergently ranked for residual methane emissions
| PRJNA797238 | ENA
Project description:comparative study of rumen microbial diversity on steer
| PRJNA527936 | ENA
Project description:Protozoa comprise a major fraction of the microbial biomass in the rumen microbiome, of which the entodiniomorphs (order: Entodiniomorphida) and holotrichs (order: Vestibuliferida) are consistently observed to be dominant across a diverse genetic and geographical range of ruminant hosts. Despite the apparent core role that protozoal species exert, their major biological and metabolic contributions to rumen function remain largely undescribed in vivo. Here, we have leveraged (meta)genome27 centric metaproteomes from rumen fluid samples originating from both cattle and goats fed diets with varying inclusion levels of lipids and starch, to detail the specific metabolic niches that protozoa occupy in the context of their microbial co-habitants. Initial proteome estimations via total protein counts and label-free quantification highlight that entodiniomorph species Entodinium and Epidinium as well as the holotrichs Dasytricha and Isotricha comprises an extensive fraction of the total rumen metaproteome. Proteomic detection of protozoal metabolism such as hydrogenases (Dasytricha, Isotricha, Epidinium, Enoploplastron), carbohydrate-active enzymes (Epidinium, Diplodinium, Enoploplastron, Polyplastron), microbial predation (Entodinium) and volatile fatty acid production (Entodinium and Epidinium) was observed at increased levels in high methane-emitting animals. Despite certain protozoal species having well-established reputations for digesting starch, they were unexpectedly less detectable in low methane emitting-animals fed high starch diets, which were instead dominated by propionate/succinate-producing bacterial populations suspected of being resistant to predation irrespective of host. Finally, we reaffirmed our abovementioned observations in geographically independent datasets, thus illuminating the substantial metabolic influence that under-explored eukaryotic populations have in the rumen, with greater implications for both digestion and methane metabolism.
2023-04-19 | PXD040454 | Pride