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RNA-Seq analysis of human osteoarthritic intact cartilage following total knee replacement

ABSTRACT: RNA-Seq analysis of human osteoarthritic intact cartilage following total knee replacement

PROVIDER: PRJEB23730 | ENA |

REPOSITORIES: ENA

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RNA-Seq analysis of human osteoarthritic intact cartilage following total knee replacement

Project description:Large scale RNA-Seq analysis was performed to investigate the transcriptomic response to osteoarthritis in cartilage and investigate potential subgroups of patients. Data were collected from intact knee cartilage (posterior lateral condyle) from at total of 60 patients with osteoarthritis (OA) following total knee replacement and 10 control non-OA patients following amputation.

2017-12-13 | E-MTAB-6266 | biostudies-arrayexpress

RNA-Seq analysis of human intact and damaged osteoarthrtitic cartilage following total knee replacement.

Project description:This experiment captures the expression of genes between two sites of human cartilage within the same patients to allow investigation of genomic responses to damage during osteoarthritis. Eight patients with symptomatic OA undergoing total knee replacement (n=8, age range 65-79 years, mean age 70.3) were used in this study. Cartilage from paired osteochondral samples were isolated from the intact PLC (posterior lateral condyle) and the damaged DMC (distal medial condyle) for RNA-seq analysis.

2016-04-06 | E-MTAB-4304 | biostudies-arrayexpress

Genome-wide DNA methylation study of hip and knee osteoarthritic and healthy cartilage reveals embryonic organ and skeletal system morphogenesis as major pathways involved in osteoarthritis

Project description:Cartilage samples were collected from hip or knee joint replacement patients either due to primary OA or hip fractures as controls. DNA was extracted from the collected cartilage and assayed by Illumina Infinium HumanMethylation450 âBeadChip array, which allows for the analysis of >480,000 CpG sites. Bisulphite converted DNA from 5 hip osteoarthritic, 6 knee osteoarthritic and 7 hip healhty cartilage samples were hybridised to the Illumina Infinium HumanMethylation450 âBeadChip array

2016-07-01 | E-GEOD-73626 | biostudies-arrayexpress

RNA-Seq analysis of human intact and damaged osteoarthrtitic cartilage following total knee replacement.

Project description:RNA-Seq analysis of human intact and damaged osteoarthrtitic cartilage following total knee replacement.

| PRJEB12436 | ENA

Differential expression analysis of human small RNAs in young normal, old normal (protected)) and old osteoarthritis (unprotected ) knee cartilage

Project description:Human cartilage taken from young normal knees following anterior cruciate ligament repair and osteoarthritic cartilage; old normal (protected) and old osteoarthritic (unprotected) following total knee arthroplasty. Total RNA extracted using mirVana miRNA isolation kit (Life Technologies). The Affymetrix Flash Tag labelling kit was used to prepare samples for hybridisation onto Affymetrix miRNA 4.0 arrays.

2017-12-31 | E-MTAB-5716 | biostudies-arrayexpress

Genome-wide DNA methylation study of hip and knee osteoarthritic and healthy cartilage reveals embryonic organ and skeletal system morphogenesis as major pathways involved in osteoarthritis

2016-07-01 | GSE73626 | GEO

Forward and reverse degradomics defines the proteolytic landscape of human knee osteoarthritic cartilage and the role of the serine protease HtrA1

2023-11-03 | PXD028060 | Pride

Differential expression analysis of small RNAs in young normal and old osteoarthritis cartilage

Project description:Human cartilage taken from young normal knees following anterior cruciate ligament repair and osteoarthritic cartilage following total knee arthroplasty. Total RNA extracted using mirVana miRNA isolation kit (Life Technologies). The Affymetrix Flash Tag labelling kit was used to prepare samples for hybridisation onto Affymetrix miRNA 4.0 arrays.

2017-12-31 | E-MTAB-5715 | biostudies-arrayexpress

Characterization of the cartilage DNA methylome in knee and hip osteoarthritis.

Project description:The aim of this study was to characterise the genome-wide DNA methylation profile of osteoathritis (OA) chondrocytes from both knee and hip cartilage, providing the first comparison of DNA methylation between OA and non-OA hip cartilage, and between OA hip and OA knee cartilage. The study was performed using the Illumina Infinium HumanMethylation450 BeadChip array. Genome-wide methylation was assesed in chondrocyte DNA extracted from 23 OA hip, 73 OA knee and 21 healthy hip controls (NOF - neck of femure samples). Keywords: Methylation profiling by array

2015-09-02 | GSE63695 | GEO

Forward and reverse degradomics defines the proteolytic landscape of human knee osteoarthritic cartilage and the role of the serine protease HtrA1

Project description:Proteolytic destruction of articular cartilage is a major pathogenic mechanism in osteoarthritis (OA), but was not previously investigated on a proteome-wide scale. We sought to define the human knee OA cartilage degradome and the contribution therein of the serine protease HtrA1, using an N-terminomics strategy, terminal amine isotopic labeling of substrates (TAILS).

2023-11-03 | PXD028320 | Pride

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