ABSTRACT: EMG produced TPA metagenomics assembly of the Metagenomes from human infant fecal samples with and without necrotizing enterocolitis (human gut metagenome) data set.
Project description:91 preterm infant gut metaproteomes measured in technical duplicate using an eleven salt pulse 2D-LC-MS/MS method. Samples represent 17 preterm infants over the first several weeks of life, of which 6 preterm infants eventually developed necrotizing enterocolitis.
Project description:Preterm infants are highly susceptible to late-onset sepsis (LOS) and necrotizing enterocolitis (NEC) but specific biomarkers for diagnosis and effective treatment are lacking. Neutrophil extracellular traps (NETs) are related to sepsis in adults but not investigated in infant conditions. This is the first proteome study to document that circulating NETs are involved in neonatal LOS and NEC. cfDNA and NET proteins may provide new potential diagnostic markers for these diseases.
2018-03-05 | PXD004082 | Pride
Project description:EMG produced TPA metagenomics assembly of the preterm infant gut metagenomes (NIH Y4 Cohort) (human gut metagenome) data set.
| PRJEB24779 | ENA
Project description:EMG produced TPA metagenomics assembly of the Skin metagenomes (human skin metagenome) data set.
| PRJEB29894 | ENA
Project description:EMG produced TPA metagenomics assembly of PRJNA237344 data set (Amazon Continuum Metagenomes).
| PRJEB46727 | ENA
Project description:EMG produced TPA metagenomics assembly of the ISS Metagenomes (PRJNA438545) data set.
Project description:Preterm neonates are susceptible to gastrointestinal (GI) disorders such as necrotizing enterocolitis (NEC). Maternal milk, and especially colostrum, protects against NEC via growth promoting, immunomodulatory and antimicrobial factors. The fetal enteral diet, amniotic fluid (AF), contains similar bioactive components and we hypothesized that postnatal AF administration would reduce inflammatory responses and NEC in preterm neonates. Thirty preterm pigs (92% gestation) were delivered by caesarean section and fed total parental nutrition (TPN) for 48 h followed by enteral porcine colostrum (COLOS, n=7), infant formula (FORM, n=13) or formula + porcine AF (AF, n=10). Using a previously validated model of NEC in preterm pigs, we determined the structural, functional, microbiological and immunological responses to AF when administered prior to and after introduction of a suboptimal enteral formula diet. Keywords: Healthy versus inflammed tissues in relation to necrotizing enterocolitis
