Ugly ducklings - The dark side of plastic materials in contact with potable water
ABSTRACT: Biofilms from the inner surface of multiple bath toys were characterised, particularly focussing on total cell counts (flow cytometry) and microbial community compositions (16S rDNA amplicon sequencing). A control study was done to elucidate the impact of (1) the flexible plastic material, (2) bacteria from the tap water, and (3) biological and chemical properties of the used bath water on these biofilms.
Project description:These experiments were designed to study the effect of heat shock and cold shock on expression profiles of a hyperthermophilic archaeon after a twenty-minute temperature shock. When cells entered the middle of the exponential growth phase at the optimal growth temperature of 85 C, they were transferred from an 85 C water bath to a 95 C water bath or a 65 C water bath. Two biological replicates were obtained under both heat shock and cold shock conditions. For each biological replicate, we performed a total of six technical replicates consisting of three pairs of flip-dye experiments. As for the reference sample, a pooled population of cDNA from three biological replicates consisting of cells growing in the mid-exponential phase at 85 C was used. All hybridizations were performed against this reference
Project description:We compared the transcriptome of stationary-phase cells with mature biofilm cultured with and without oxygen. The reads were mapped to the genome and then normalized counts for each gene were calculated, thus enabling the comparison of gene-expression levels between samples. Our results depicts the severe stress the lack of oxygen imposes on biofilms by altered gene expression mRNA profiles of aerobic and anaerobic biofilm and planktonic cultures were generated by deep sequencing
Project description:Marinobacter hydrocarbonoclasticus SP17 strain forms biofilms on water-insoluble hydrophobic organic compounds which it can use as the sole source for carbon and energy. Gene expression profiles were determined in biofilm cells grown on hexadecane (an alkane), triolein (a triglyceride) and planktonic cells growing exponentially on acetate (a water-soluble organic acid). Three independent biological replicates were used for each condition.
Project description:Metagenomic and metaproteomic analyses were utilized to determine the composition and function of complex air-water interface biofilms sampled from the hulls of two ships that were deployed in different geographic locations.
Project description:Experimental factor: Time after the temperature shift from 30C to 37C or 30C to 48C. Conidia (5x106 /ml) from Aspergillus fumigatus Af293 were incubated in the CM medium for germination (~ 17 hrs) at 30C. The culture was transferred to a water bath of 37C or 48C for continued growth. Samples were taken after the defined time (i.e. 0, 15, 30, 60, 120, and 180 min.).