Project description:The microsporidia Nosema ceranae are intracellular parasites that proliferate in the midgut epithelial cells of honey bees (Apis mellifera). To analyze the pathological effects of those microsporidia, we orally infected honey bee workers 7 days after their emergence. Bees were flash frozen 15 days after the infection. Then, the effects on the gut ventriculi were analyzed and compared to non-infected (control) bees. Overall design: Comparisons of control vs Nosema ceranae bees
Project description:Background: Honey bee is a major insect used for pollination of a number of commercial crops worldwide. However, the number of managed honey bee colonies has recently declined in several countries, and a number of possible causes are proposed. Although the use of honey bees for pollination can be considered as disruption of the habitat, its effects on honey bees' physiology have never been addressed. In Japan, more than 100 thousands colonies are annually used for pollination, and intriguingly 80% of them are used in greenhouses. Recently, honey bee colonies have often collapsed when they are introduced into greenhouses. Thus, to suppress colony collapses and maintain the number of worker bees in the colonies are essential for successful long-term pollination in greenhouses and recycling honey bee colonies. Honey bee hives were installed into strawberry and eggplant greenhouses, and then the gene expression profiles of the honey bees were examined at the different time periods. Total 16 samples with two replicates were analyzed.
Project description:In this study we addressed whether the transcriptome profile in the honey bee brain is similar for two major parasites of honey bee, Varroa destructor and Nosema ceranae. Honey bees parasitized by these two parasites show accelerated behavioral maturation and deficiences in orientation and learning/memory that we hoped to characterized at the transcriptomic level. honey bee adults infested by Varroa destructor or Nosema ceranae compared to control bees, in duplicate
Project description:Manuka honey has been shown to inhibit growth in EMRSA-15 by inhibiting cell division, the mode of actin is currently unclear. We used microarrays to detail the difference in global gene expresson following treatment of cells with honey. We identified distinct classes of up and down regulated genes EMRSA-15 were harvested 4 hours after exposure to treatment for RNA extraction and hybridization on Affymetrix microarrays. Control and honey treated cells were investigates, three biological replicates were used for each sample.
Project description:Manuka honey has been shown to inhibit growth in Pseudomonas aueruginosa, the mode of action is currently unclear. We used microarrays to detail the difference in global gene expresson following treatment of cells with honey. We identified distinct classe of up and down regulated genes Pseudomonas aueruginosa were harvested 4 hours after exposure to treatment for RNA extraction and hybridization on Affymetrix microarrays. Control and honey treated cells were investigates, three biological replicates were used for each sample.
Project description:Honey bee non-CG DNA hydroxymethylation is enriched in the introns, which supplements previous findings that honey bee CG DNA methylation is enriched in exons. Bisulfite sequencing combined with Pvu-Seq to distinguish 5-methylcytosine from 5-hydroxymethylcytosine and RNA-Seq