Project description:Aconitate decarboxylase 1 (ACOD1) is the enzyme synthesizing itaconate, an immuno-regulatory metabolite tuning host-pathogen interactions. Such functions are achieved by affecting metabolic pathways regulating inflammation and microbe survival. However, at the whole-body level, metabolic roles of itaconate remain largely unresolved. By using multiomics-integrated approaches, here we show that ACOD1 responds to high-fat diet consumption in mice by promoting gut microbiota alterations supporting metabolic disease. Genetic disruption of itaconate biosynthesis protects mice against obesity, alterations in glucose homeostasis and liver metabolic dysfunctions by decreasing meta-inflammatory responses to dietary lipid overload. Mechanistically, fecal metagenomics and microbiota transplantation experiments demonstrate such effects are dependent on an amelioration of the intestinal ecosystem composition, skewed by high-fat diet feeding towards obesogenic phenotype. In particular, unbiased fecal microbiota profiling and axenic culture experiments point towards a primary role for itaconate in inhibiting growth of Bacteroidaceae and Bacteroides, family and genus of Bacteroidetes phylum, the major gut microbial taxon associated with metabolic health. Specularly to the effects imposed by Acod1 deficiency on fecal microbiota, oral itaconate consumption enhances diet-induced gut dysbiosis and associated obesogenic responses in mice. Unveiling an unrecognized role of itaconate, either endogenously produced or exogenously administered, in supporting microbiota alterations underlying diet-induced obesity in mice, our study points ACOD1 as a target against inflammatory consequences of overnutrition.
2024-02-05 | GSE213632 | GEO
Project description:EMG produced TPA metagenomics assembly of PRJNA479723 data set (Nanopore metagenomics).
Project description:This study aimed to analyze changes in gut microbiota composition in mice after transplantation of fecal microbiota (FMT, N = 6) from the feces of NSCLC patients by analyzing fecal content using 16S rRNA sequencing, 10 days after transplantation. Specific-pathogen-free (SPF) mice were used for each experiments (N=4) as controls.
Project description:EMG produced TPA metagenomics assembly of PRJNA851847 data set (Mouse gut microbiota macrogenome).
| PRJEB54774 | ENA
Project description:EMG produced TPA metagenomics assembly of PRJNA728379 data set (Metagenome of pig fecal bacteria).
| PRJEB52635 | ENA
Project description:EMG produced TPA metagenomics assembly of PRJEB25419 data set (Breast Cancer Metagenomics).
| PRJEB51311 | ENA
Project description:EMG produced TPA metagenomics assembly of the Sheep faecal microbiota (Sheep faecal microbiota) data set.
| PRJEB26094 | ENA
Project description:EMG produced TPA metagenomics assembly of the PRJNA357148 data set (Cow-to-mouse fecal transplantation established intestinal microbiota as cause of cow mastitis).