Project description:Single-cell RNA-seq of human dermal fibroblasts, stimulated with IFNB 1000 IU for 2 or 6 hours, and profiled using the SmartSeq2 protocol.
Project description:Dermal fibroblasts from human, stimulated with dsRNA (poly I:C) in a time course of 0, 2, 6 hours, profiled using the Smart-seq2 protocol
Project description:We report the responsiveness of bovine dermal fibroblasts to lipopolyssacharide (LPS) within individuals at different ages. Cultures were collected from three different heifers at the ages of approximately 5 and 16 months. Isolated fibroblasts were then exposed to LPS to determine whether there was a differential response within an animal based upon age.
Project description:The goal of this project is to compare the protein composition of the extracellular matrices (ECMs) deposited in vitro by wild type, heterozygous, or SPAG17 KO neonatal mouse dermal fibroblasts.
Project description:Dermal fibroblasts from one human individual were stimulated with dsRNA (poly I:C) in a time course of 2,4 and 6 hours. In one time course - the dsRNA was removed after 30mins, in a second time course it was removed after 1 hour, while in the third the dsRNA was kept in medium for the entire time course. Following collection, cells were profiled using the Smart-seq2 protocol.
Project description:We report the responsiveness of bovine dermal fibroblasts to lipopolyssacharide (LPS) within individuals at different ages. Cultures were collected from three different heifers at the ages of approximately 5 and 16 months. Isolated fibroblasts were then exposed to LPS to determine whether there was a differential response within an animal based upon age. Fibroblasts were isolated from 3 cows at the ages 5 months and 16 months. Isolated fibroblasts were treated with LPS for 0 hrs, 2 hrs, and 8 hrs.