Project description:Complete transcriptome assembly and annotation of a critically important amphipod species in freshwater ecotoxicological risk assessment: Gammarus fossarum

| PRJNA556212 | ENA

Egg yolk proteins G. fossarum - High-throughput proteome dynamics for discovery of key proteins in sentinel species: Unsuspected vitellogenins diversity in the crustacean Gammarus fossarum

Project description:We aimed to specifically identify the major egg yolk proteins (i.e. vitellogenin) in the amphipod Gammarus fossarum, a sentinel species in freshwater risk assessment. Vitellogenin production in female is vital for the embryonic development and its production in male organism is commonly employed as a biomarker of exposition to xenoestrogens in fish. We performed a shotgun proteomics analysis on embryos and ovaries at different stages of their development. The proteome dynamics over the cycle was analyzed to correlate on the first hand proteins which are accumulating along vitellogenesis and on the other hand, proteins with decrease amounts long embryogenesis. This information led to the proposal of putative novel VTG candidates. Our data provides the first large scale molecular description of a crustacean reproductive cycle, while our temporal analysis evidenced seven candidates proteins as “true vitellogenin” in G. fossarum.

2016-07-15 | PXD001002 | Pride

Proteogenomic analysis of hemolymph from Gammarus fossarum

Project description:Proteome profiles of hemolymph from Gammarus fossarum established by next-generation proteomics

2023-09-15 | PXD031777 | Pride

Male infertility G. fossarum - While numerous examples of male reproductive disorders have been reported in vertebrates, invertebrate’s organisms have been considerably less studied, despite their ecological importance

Project description:While numerous examples of male reproductive disorders have been reported in vertebrates, invertebrate’s organisms have been considerably less studied, despite their ecological importance. The aim of this study is to investigate male infertility in the amphipod Gammarus fossarum, a sentinel species in freshwater risk assessment. Thus in laboratory, we exposed male gammarids to different concentrations of three different xenobiotics: cadmium, and two potent arthropods endocrine-disruptor chemicals, methoxyfenozide and pyriproxyfen. Afterward, we investigated alterations of reproductive health by sperm quality markers and proteomes dynamics on the male reproductive tissue by nanoLC-MS/MS for evidencing proteins modulated by toxic exposure.

2014-11-04 | PXD001267 | Pride

Proteogenomic analysis of gills and caeca from Gammarus fossarum

Project description:Proteome profiles of gills and caeca from three Gammarus fossarum males established by next-generation proteomics

2023-09-15 | PXD040344 | Pride

Proteomic response of Gammarus fossarum subjected to dietary methyl-mercury

Project description:Proteogenomics analysis from Gammarus fossarum exposed to dietary methyl-mercury informed by RNA-seq derived database.

2023-02-04 | PXD024583 | Pride

Proteomics of Gammarus fossarum A individuals sampled from the wild

Project description:Next-generation proteomics of Gammarus fossarum A animals sampled from the Seebach river in north-eastern France.

2020-04-17 | PXD014166 | Pride