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Streptococcus pyogenes strain S119

ABSTRACT: Genome sequence of Streptococcus pyogenes strain S119

PROVIDER: PRJEB27939 | ENA |

REPOSITORIES: ENA

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	ERR2715044.fastq.gz	Fastqsanger.gz

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RNA-seq to investigate differential expression of serotype emm1 Streptococcus pyogenes strains S119 and S126

Project description:The human pathogen Streptococcus pyogenes, or group A streptococcus, is responsible for mild infections to life-threatening diseases. We previously have performed the transcription start site profiling of a Streptococcus pyogenes emm1 strain, strain S119, an invasive strain isolated from a blood culture. Here, we perform strand-specific RNA-seq experiments to complete this characterization and analyze the global coverage and the differential expression in growth medium complemented or not with 15 mM MgCl2. In addition we compare these results to those obtained with a related strain, strain S126, corresponding to a colonization sample, that differs from S119 by only one mutation in the two-component regulator of virulence CovRS.

2018-11-30 | E-MTAB-7101 | biostudies-arrayexpress

Transcription start site profiling of a Streptococcus pyogenes emm1 strain

Project description:The human pathogen Streptococcus pyogenes, or group A streptococcus, is responsible for mild infections to life-threatening diseases. To determine the primary transcriptome of the emm1 strain S119, we have performed a differential RNA-Seq experiment based on selective Tobacco Acid Pyrophosphatase (TAP) treatment and 5' adapter ligation to differentiate primary transcripts (5' tri-phosphate) and processed RNAs (5' mono-phosphate). The libraries were performed on a mixture of RNAs prepared from bacteria cultured to late exponential phase in a rich growth culture medium supplemented or not with 15 mM of MgCl2

2018-11-30 | E-MTAB-7099 | biostudies-arrayexpress

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