Project description:We used 10X Genomics Chromium system to profile single cells from a pool of Wild-type Ciona robusta embyros at 12 hours post fertilization (hpf)

2021-05-10 | GSE174127 | GEO

Ciona intestinalis

Project description:Ciona intestinalis, Ciona roulei, and Ciona robusta raw sequence reads from ddRADseq

| PRJNA759193 | ENA

Ciona robusta

Project description:A microRNA-mRNA Expression Network during Oral Siphon Regeneration in Ciona [microRNA]

| PRJNA335345 | ENA

Ciona robusta

Project description:A microRNA-mRNA Expression Network during Oral Siphon Regeneration in Ciona [mRNA]

| PRJNA335346 | ENA

Ciona robusta

Project description:Gene expression profiling in Ciona TVCs with FGF-MAPK perturbation [bulk RNA-seq]

| PRJNA389948 | ENA

Single-cell transcriptome profiling of the Ciona larval brain

Project description:The tadpole-type larva of Ciona has emerged as an intriguing model system for the study of neurodevelopment. The Ciona intestinalis connectome has been recently mapped, revealing the smallest central nervous system (CNS) known in any chordate, with only 177 neurons. This minimal CNS is highly reminiscent of larger CNS of vertebrates, sharing many conserved developmental processes, anatomical compartments, neuron subtypes, and even specific neural circuits. Thus, the Ciona tadpole offers a unique opportunity to understand the development and wiring of a chordate CNS at single-cell resolution. Here we report the use of single-cell RNAseq to profile the transcriptomes of single cells isolated by fluorescence-activated cell sorting (FACS) from the whole brain of Ciona robusta (formerly intestinalis Type A) larvae. We have also compared these profiles to bulk RNAseq data from specific subsets of brain cells isolated by FACS using cell type-specific reporter plasmid expression. Taken together, these datasets have begun to reveal the compartment- and cell-specific gene expression patterns that define the organization of the Ciona larval brain.

2018-10-26 | GSE121807 | GEO

An Atlas of the Binding Specificities of Transcription Factors in Pseudomonas aeruginosa Directs Prediction of Novel Regulators in Virulence

Project description:We performed a high-throughput systematic evolution of ligands by exponential enrichment (HT-SELEX) approach on all 371 annotated TFs in P. aeruginosa. This study provides a valuable resource for TF binding specificities in P. aeruginosa and demonstrates a novel and an integrative analysis for seeking the virulence-associated TFs and its target genes.

2021-03-26 | GSE151518 | GEO

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