Project description:Late blight, caused by the oomycete Phytophthora infestans, is one of the most damaging potato diseases. Genetic resistance is one of the most effective means to control the destruction caused by this pathogen. Transgenic potato lines harboring a resistance gene, RB, confer broad-spectrum, rate-reducing late blight resistance. A microarray approach was used to understand what genes are manipulated in the potato background after the addition of the RB gene that contribute to the late blight resistant phenotype. Keywords: Time course, disease state analysis
Project description:Late blight, caused by the oomycete Phytophthora infestans, is one of the most damaging potato diseases. Genetic resistance is one of the most effective means to control the destruction caused by this pathogen. Transgenic potato lines harboring a resistance gene, RB, confer broad-spectrum, rate-reducing late blight resistance. A microarray approach was used to understand what genes are manipulated in the potato background after the addition of the RB gene that contribute to the late blight resistant phenotype. Keywords: Time course, disease state analysis CRD (3x2x2) Split-Split Plot: 3 sampling time points after inoculation (2, 5, 10 hours), Two genotypes (Katahdin with and without the RB gene), Inoculation with P. infestans or mock inoculation with water. 48 arrays were hybridized in total; 12 in each biological replicate. Each genotype with the mock and late blight inoculated samples was hybridized on two arrays using a dye-swap procedure. Each genotype had a total of 6 arrays across the three sampling time points.
Project description:The project aimed to study the secretome profile of the Goss's bacterial wilt and blight disease of corn caused by the phytobacterial pathogen Clavibacter nebraskensis (Cn). The experiment was carried out in Vitro using two Cn isolates (A highly aggressive isolate Cmn14-5-1 and a weakly aggressive DOAB232) from Manitoba, Canada grown in two different xylem sap media (Host xylem sap from corn (CXS), and non-host xylem sap from tomato (TXS), besides M9 medium.
Project description:We carried out an RNA-seq based transcriptome study on two rice varieties, Cocodrie (CCDR; rice sheath blight susceptible) and MCR10277 (MCR; rice sheath blight resistant), to profile the time-series wide genome-scale transcriptional differences in response to sheath blight (SB), an infection caused by R. solanii (LR172) . Our approach is cross-referencing differentially expressed genes with significant variants of two phenotypically different varieties to validate known and discover novel variants and to further understand rice's physiological response to SB.
Project description:Seedlings blight is one of the destructive diseases of rice, caused by the fungus Fusarium oxysporum, which impairs rice productions. Fluoro-substituted benzothiadiazole derivatives (FBT) and chitosan oligosaccharide (COS) are elicitors that can enhance plant resistance to pathogen infection. However, there is a lack of information regarding FBT and COS used as elicitors in rice seedlings blight. Therefore, the aim of this study was to evaluate the effect of FBT and COS treatments against rice seedling blight and elucidate the possible molecular mechanisms of the two elicitors for inducing resistance by proteomics. The results showed that FBT and COS significantly reduced the disease incidence and index, and improved the growth status of rice root caused by F. oxysporum. Biochemical analysis demonstrated that the two elicitors effectively enhanced activities of defense enzymes. Moreover, proteomic profiling analysis of rice root tissues disclosed more differentially expressed proteins in diterpenoid biosynthesis pathway induced by the two elicitors compared with other biological pathways, resulting in the accumulation of antimicrobial substance--momilactone. This study provided the basis of theory and application for FBT and COS used as rice elicitors against seedling blight on roots.
