RNA-Seq of chicken breast samples treated with chronic heat stress
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ABSTRACT:
PROVIDER: PRJEB30126 | ENA |
REPOSITORIES: ENA
Similar Datasets
Project description:To study the effect of chronic heat stress on producing laying type hens, RNA-Seq was performed on breast samples during a 4-week heat stress experiment. RNA-Seq samples were collected 3-hour, 2-weeks, and 4-weeks post heat from both control (unheated) and treatment (heated) birds. RNA was extracted from the tissues with RNAqueous Total RNA Isolation Kit, treated with DNase, and mRNA cDNA libary were prepared with Illumina TrueSeq Stranded mRNA Library Prep Kit. Sequencing was done on 4 lanes with 12 libraries per lane on Illumina HiSeq 3000 platform.
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Project description:RNA-Seq of chicken cecal tonsil samples treated with chronic heat stress
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Project description:To study the effect of chronic heat stress on mature laying type hens, RNA-Seq was performed on cecal tonsil samples during a 4-week heat stress experiment. RNA-Seq samples were collected 3-hour, 2-weeks, and 4-weeks post heat from both control (unheated) and treatment (heated) birds. RNA was extracted from the tissues with RNAqueous Total RNA Isolation Kit, treated with DNase, and mRNA cDNA libary were prepared with Illumina TrueSeq Stranded mRNA Library Prep Kit. Sequencing was done on 2 lanes with 12 libraries per lane on Illumina HiSeq 3000 platform.
2019-05-01 | E-MTAB-7865 | biostudies-arrayexpress
Project description:By using a 44k chicken Agilent microarray, we systematically analyzed the chicken hypothalamus transcriptome response to thermal stress. Twelve hypothalamus samples were chosen from three groups (four samples per group) to be used in chicken genome microarray to examine differential gene expression.We compared the expression profiles between each pairs of the three groups using the microarray data. Totally, 2474 genes were found to be differentially expressed in the three comparisons with p＜0.05 and fold change (FC) higher than 1.5 and the genes were mainly involved in self-regulation and compensation required to maintain homeostasis, including heat shock protein family, enzyme and the hormone, neurotransmitter, cell-cell signaling, metabolism and cytokines. The transcripts of heat shock protein including Hsp 40 and Hsp 90 were significantly changed respond to thermal stress and genes involved in regulation of cell morphogenesis were significantly upregulated in heat stressed group with comparison to control and temperature recovery group. Additionally, the down-regulated genes in both heat stress and temperature recovery groups compared to control group were enriched in muscle organ development, striated muscle tissue development, cardiac muscle tissue development and muscle tissue development, which indicates that muscle development was inhibited during and in short-term after heat treatment. Most of genes dysregulated in heat stress group were found to be recovered in temperature recovery group, which confirmed their roles they could play in coping with heat stress. The present study provides a broader understanding of molecular mechanisms underlying the stress response in chicken and discovery of novel genes that are regulated in a thermal stress specific manner.
2012-10-01 | GSE37400 | GEO
Project description:The liver plays a central role in metabolism and is important in maintaining homeostasis throughout the body. This study integrated transcriptomic and metabolomic data to understand how the liver responds under chronic heat stress. Chickens from a rapidly growing broiler line were heat stressed for 8 hours per day for one week and liver samples were collected at 28 days post hatch. Transcriptome analysis reveals changes in genes responsible for cell cycle regulation, DNA replication, and DNA repair along with immune function. Integrating the metabolome and transcriptome data highlighted multiple pathways affected by heat stress including glucose, amino acid, and lipid metabolism along with glutathione production and beta- oxidation.
2017-02-21 | GSE95088 | GEO
Project description:Heat stress is a very complex physiological process. At present, there are some reports about physiological function and pathological changed on chicken heat stress. We use DNA microarrays to detail the global programme of gene expression underlying heat stress and identified distinct classes of differentially expressed (DE) genes during this process.
2010-12-31 | GSE23592 | GEO
Project description:By using a 44k chicken Agilent microarray, we systematically analyzed the chicken hypothalamus transcriptome response to thermal stress. Twelve hypothalamus samples were chosen from three groups (four samples per group) to be used in chicken genome microarray to examine differential gene expression.We compared the expression profiles between each pairs of the three groups using the microarray data. Totally, 2474 genes were found to be differentially expressed in the three comparisons with pM-oM-<M-^\0.05 and fold change (FC) higher than 1.5 and the genes were mainly involved in self-regulation and compensation required to maintain homeostasis, including heat shock protein family, enzyme and the hormone, neurotransmitter, cell-cell signaling, metabolism and cytokines. The transcripts of heat shock protein including Hsp 40 and Hsp 90 were significantly changed respond to thermal stress and genes involved in regulation of cell morphogenesis were significantly upregulated in heat stressed group with comparison to control and temperature recovery group. Additionally, the down-regulated genes in both heat stress and temperature recovery groups compared to control group were enriched in muscle organ development, striated muscle tissue development, cardiac muscle tissue development and muscle tissue development, which indicates that muscle development was inhibited during and in short-term after heat treatment. Most of genes dysregulated in heat stress group were found to be recovered in temperature recovery group, which confirmed their roles they could play in coping with heat stress. The present study provides a broader understanding of molecular mechanisms underlying the stress response in chicken and discovery of novel genes that are regulated in a thermal stress specific manner. Hypothalamus samples were collected from non-heat treated group (reared at 25C, used as control), 24h 34C treated group (heat stress treated group) and temperature recovery group (25C for 24h followed heat stress).
2012-10-01 | E-GEOD-37400 | biostudies-arrayexpress
Project description:This study was done to know the transcritional changes occuring in mice liver in response to chronic heat stress at 34*c for a period of two weeks. Keywords: Chronic heat stress response on liver gene expression.
2005-10-24 | GSE2985 | GEO