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MRNA-seq of mouse salivary gland cancer cells cultured in adherent or non-adherent culture

ABSTRACT: mRNA-seq of mouse salivary gland cancer cells cultured in adherent or non-adherent culture

PROVIDER: PRJEB30356 | ENA |

REPOSITORIES: ENA

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mRNA-seq of mouse salivary gland cancer cells cultured in adherent or non-adherent culture

Project description:Aim: investigate how the Wnt-driven Mll1 epigenome regulates salivary gland and head and neck cancer. We performed mRNA-seq and ChIP-seq of H3K4me1, me2 and me3 on mouse salivary gland cancer cells that are kept in two different growth conditions, adherent culture and non-adherent sphere culture. Mouse salivary gland cancer cells were isolated from salivary gland of transgenic mouse that harbor K14-Cre-induced Wnt/β-catenin gain-of-function and Bmpr1a loss-of-function mutations.

2018-12-24 | E-MTAB-7509 | biostudies-arrayexpress

ChIP-seq of H3K4me1, me2 and me3 on mouse salivary gland cancer cells cultured in either adherent or non-adherent conditions

Project description:Investigating how the Wnt-driven Mll1 epigenome regulates salivary gland and head and neck cancer. We performed mRNA-seq and ChIP-seq of H3K4me1, me2 and me3 on mouse salivary gland cancer cells that are kept in two different growth conditions, adherent culture and non-adherent sphere culture. Mouse salivary gland cancer cells were isolated from salivary gland of transgenic mouse that harbor K14-Cre-induced Wnt/β-catenin gain-of-function and Bmpr1a loss-of-function mutations. Anti-H3K4me1 (C15410194), -me2 (C15410035) and -me3 (C15410003-50) antibodies were purchased from Diagenode. ChIP-seq was performed according to the protocols provided by Diagenode using the iDeal ChIP-seq kit for histones and the iDeal library preparation kit. For mRNA-seq, mRNA was extracted according to the standard TRIzol protocol (Invitrogen) and subjected to library preparation using the TruSeq stranded mRNA library preparation kit. Sequencing was performed with the TruSeq SBS Kit v3-HS (2 X 200 cycles) on an Illumina HiSeq 2000 sequencer.

2018-12-24 | E-MTAB-7514 | biostudies-arrayexpress

ChIP-seq of H3K4me1, me2 and me3 on mouse salivary gland cancer cells cultured in either adherent or non-adherent conditions

Project description:ChIP-seq of H3K4me1, me2 and me3 on mouse salivary gland cancer cells cultured in either adherent or non-adherent conditions

| PRJEB30353 | ENA

Salivary gland cancer organoids are valid for preclinical genotype-oriented medical precision trials

Project description:We established patient-derived organoids and monolayer culture cells from the salivary gland cancer cases. To compare the RNA profiles of primary culture cells (Organoids and monolayer culture cells) and their parental tumors, we isolated total RNA from 2 cases of the salivary gland cancer and performed transcriptome sequencing for the organoids, monolayer culture cells, and their parental tumors of both cases. Case 6 is a case of adenoid cystic carcinoma and Case 11 is a case of salivary duct carcinoma.

2023-04-27 | GSE223554 | GEO

CaSki cells: adherent cultured cell vs. sphere cells

Project description:Transcriptional profiling of human cervical squamous cell carcinoma cell line CaSki comparing adherent cultured cells with spheres cultured in serum free culture medium. Goal was to identify candidate antigens for immunotherapy targeting cancer stem cells.

2015-03-04 | GSE65499 | GEO

RNA-seq data for Huh7 cells in spheroid culture and adherent culture

Project description:Anchorage-independent spheroid cells of HCC possess stemness properties. Huh7 cells are cultured in ultra-low attachment surface plates with serum-free medium. RNA-sequencing (RNA-seq) was applied in spheroid culture cells and adherent culture cells.

2022-06-01 | GSE199940 | GEO

Gene expression of murine 3 major salivary gland organoids

Project description:Salivary glands that produce and secret saliva, which is essential for lubrication, digestion, immunity, and oral homeostasis, consist of diverse cells. The long-term maintenance of diverse salivary gland cells in organoids remains problematic. Here, we established long-term murine salivary gland organoids from 3 major salivary glands, including parotid gland (PG), submandibular gland (SMG), and sublingual gland (SLG). Murine salivary gland organoids expressed gland-specific genes and proteins of acinar, myoepithelial, and duct cells. Organoids were maintained in growth media (named GEM) and further underwent differentiation in differentiation media (named DAM). Our study will provide an experimental platform for the exploration of mechanisms involvled in tissue regeneration, development, or several salivary gland diseases.

2022-05-05 | GSE184091 | GEO

Transcriptional profiing of cultured adherent and non-adherent monocytes

Project description:Transcriptional profiing of cultured adherent and non-adherent monocytes

| PRJNA679607 | ENA

Human colorectal cancer cell line CRC#21: Sphere-cultured cells vs. Adherent-cultured cells

Project description:Transcriptional profiling of cancer stem cells (sphere-cultured cells) comparing non-cancer stem cells (adherent-cultured cells). Goal was to identity cancer stem cell-specific genes. Two-condition experiment, sphere-cultured cells vs. adherent-cultured cells: 1 sphere-cultured replicate and 1 adherent-culture replicate.

2016-04-01 | E-GEOD-77150 | biostudies-arrayexpress

The role of IRF6 in salivary gland development

Project description:Loss of Irf6 leads to disruption of branching morphogenesis and secretory acnii formation in salivary gland. To determine the differentially expressed genes in Irf6 mutant, embryonic salivary gland tissues were extracted at E14.5.

2017-07-29 | GSE102020 | GEO

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