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RNA-Seq of Bovan brown chicken posterior forewing regions from control and stage HH19 cyclopamine-treated samples, posterior foreleg regions of control Bovan brown and feathered Pekin bantams collected at E10.5

ABSTRACT: RNA-Seq of Bovan brown chicken posterior forewing regions from control and stage HH19 cyclopamine-treated samples, posterior foreleg regions of control Bovan brown and feathered Pekin bantams collected at E10.5

PROVIDER: PRJEB30368 | ENA |

REPOSITORIES: ENA

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RNA-Seq of Bovan brown chicken posterior forewing regions from control and stage HH19 cyclopamine-treated samples, posterior foreleg regions of control Bovan brown and feathered Pekin bantams collected at E10.5

Project description:This experiment was designed to investigate how inhibition of Shh signalling result in loss of flight feathers, and to identify genes involved in flight feather development. We carried out cyclopamine treatment of wing buds at HH19 which gives rise to 2 digit wings with absence of flight feathers. The posterior forewing regions of control and cyclopamine treated wings were analysed at E10.5, the developmental stage at which flight feathers first become apparent. Control leg tissue was also included in the analysis to enrich data for genes involved in general feather development. To enrich further for genes with roles in flight feather development, we also included samples from the feathered legs of Pekin bantam chickens. 3 biological replicates of pooled wing/leg sections were analysed and 12 samples were sequenced.

2019-12-04 | E-MTAB-7520 | biostudies-arrayexpress

E-MTAB-7520

Project description:RNA-Seq of Bovan brown chicken posterior forewing regions from control and stage HH19 cyclopamine-treated samples, posterior foreleg regions of control Bovan brown and feathered Pekin bantams collected at E10.5

2019-01-16 | E-MTAB-7520 | ExpressionAtlas

RNA-sequencing with micro-dissected boundary organoid into anterior, posterior, and boundary regions

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Whole Retina RNAseq after injury and Cyclopamine(C) treatment in zebrafish

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Transcription profiling of Xenopus expoised to cyclopamine to investigate effects on limb development

Project description:Study of the tetrapod limb has contributed a great deal to our understanding of developmental pathways and how changes to these pathways affect morphology. Most data on tetrapod limb development is known from amniotes, with far less known about genetic mechanisms of limb development in amphibians. To better understand the mechanisms of limb development in anuran amphibians, we use cyclopamine to inhibit Hedgehog signaling at various stages of limb development in Xenopus. We use transcriptomic analysis following cyclopamine exposure to understand the downstream effects of Hedgehog inhibition on gene expression. We find many aspects of Hedgehog function appear to be conserved with respect to amniotes, including the responses of ptc genes, gremlin, bmp2, and the autoregulatory property of shh. We show that, as was proposed based on experiments in chick, Sonic hedgehog plays two distinct roles in limb development â specification of digit number and specification of digit identity. In contrast to these points of conservation, we find that Hedgehog signaling is required for the maintenance of early limb bud outgrowth in Xenopus, a requirement not known for any other tetrapod. Experiment Overall Design: Eight microarray experiments were performed using RNA extracted from batches of hindlimbs dissected from Xenopus laevis tadpoles. Four of these were experimental groups exposed to cyclopamine at 1 Âµg/ml for 24 hours before animal fixation, and four were experimental groups exposed to the ethanol, the cyclopamine vehicle, at the same concentration used for cyclopamine vehicle for 24 hours. Each pool of RNA consisted of material from 18-22 hindlimb buds. Two experimental groups and two control groups were from one clutch of tadpoles, and the other four pools were from another clutch. Both samples within each of four paired groups (experimental vs. control) were exposed and processed simultaneously.

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Sonic Hedgehog inhibitor cyclopamine effect on JAR cells

Project description:Physiologically, trophoblast progenitor cells differentiate into placental villous cytotrophoblast cells (CTBs). CTBs either differentiate into invasive lineage to yields extravillous cytotrophoblast cells (EVTs), or undergo cell fusion lineage to yields syncytiotrophoblast cells (STBs)，Sonic hedgehog (Shh) together with indian hedgehog (Ihh) and desert hedgehog (Dhh) consist of ligand of hedgehog signaling pathway, which plays pivotal roles in regulating cell proliferation, cell differentiation, organogenesis and development, even involving in tumorigenesis and progression. previous study had summarized and indicatedthat hedgehog proteins played important roles in regulating hematopoiesis, vasculogenesis and angiogenesis during embryogenesis and development. Herein, we investigate the effect of the Sonic Hedgehog morphogen inhibitor Cyclopamine on JAR cells

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