Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Drought treatment and ABA feeding to ABA insensitive Populus tremula × tremuloides lines

ABSTRACT: Drought treatment and ABA feeding to ABA insensitive Populus tremula × tremuloides lines

PROVIDER: PRJEB30672 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Similar Datasets

Drought treatment and ABA feeding to ABA insensitive Populus tremula × tremuloides lines

Project description:4 weeks old rooted plantlets (P. tremula × tremuloides) of wildtype (T89) and 35S::abi1-1 lines (line 76-1 and line 76-3) were potted in soil in 1.5 l pots and were kept in a chamber with 1000 ppm CO2 supplement. The plants were grown for six weeks and well irrigated before the treatment started. Three treatments of well-watered (control), ABA feeding and drought stress were applied. Woody samples were collected after 4 weeks of treatment for RNA extraction and RNA sequencing.

2019-01-20 | E-MTAB-7588 | biostudies-arrayexpress

24-hour herbivory exposure of VHb-expressing Populus tremula x Populus tremuloides

2013-04-24 | GSE46331 | GEO

Drought treatment of Populus tremula × tremuloides with suppressed PICKLE or FDL2 gene expression

Project description:4 weeks old rooted plantlets (P. tremula × tremuloides) of wildtype (T89), PICKLE:RNAi (line 417-4 and 417-17) and FDL2:RNAi (line 510-12 and 510-18) were potted in soil in 1.5 l pots and were kept in a glass chamber. The plants were grown for eight weeks and well irrigated before the treatment started. Well-watered and drought stress treatments were applied. Woody samples were collected after 4 weeks of treatment for RNA extraction and RNA sequencing.

2019-01-20 | E-MTAB-7589 | biostudies-arrayexpress

Transcription profiling of Populus tremuloides cell suspension cultures to methyl jasmonate feeding

Project description:Gene expression response of Populus cell cultures subjected to methyl jasmonate feeding was analyzed using the Affymetrix poplar genome microarrays. Experiment Overall Design: Populus tremuloides suspension cells wereÂ treated with methyl jasmonate (MJ) in DMSO orÂ with DMSO alone (C).Â Cells were harvested after 48 hours.Â Two biological replicates were obtained for each condition.

2009-12-14 | E-GEOD-16773 | biostudies-arrayexpress

24-hour herbivory exposure of VHb-expressing Populus tremula x Populus tremuloides

Project description:The expression of stress-related genes induced by feeding of chestnut moth larvae (Conistra vaccinii L.) was studied with Vitreoscilla hemoglobin-expressing (VHb) and non-transgenic hybrid aspen lines (Populus tremula x P. tremuloides). Besides the herbivore-injured leaves (L1), cDNA microarray analyses were conducted using uninjured leaves of hybrid aspen lines positioned above (A) and below (B) the herbivory exposed leaves. Two-condition experiment, control vs. herbivory exposure. Two hybrid aspen lines: non-transgenic V617 and VHb expressing V617 /45. Of each plant, three leaf types were analysed: the injured/uninjured leaf (L1) and nonorthostichous leaf positioned above (A) and below (B). Biological replicates: 3. On each array, two samples representing L1, A or B leaf type of control and herbivory treatment of either V617 or V617/45 line. line V617: wt_A_rep1-3, wt_B_rep1-3, wt_L1_rep1-3 line V617/45: VHb_A_rep1-3, VHb_B_rep1-3, VHb_L1_rep1-3 leaf type A: wt_A_rep1-3, VHb_A_rep1-3 leaf type B: wt_B_rep1-3, VHb_B_rep1-4 leaf type L1: wt_L1_rep1-3, VHb_L1_rep1-5

2013-04-24 | E-GEOD-46331 | biostudies-arrayexpress

Identification of mRNAs Regulated in Response to Transcriptional Activation of the Arabidopsis ABA INSENSITIVE GROWTH 1 (ABIG1) Transcription Factor cDNA

Project description:Liquid grown, seven day old wild type Arabidopsis seedlings and seedlings carrying a cDNA encoding the ABA INSENSITIVE GROWTH 1 (ABIG1) gene under the control of an estrogen inducible promoter were treated with estrogen for 0, 60 and 120 minutes at which point seedlings were flash frozen and mRNA was extracted.

2016-06-22 | GSE70100 | GEO

HvABI5 is involved in ABA-dependent drought response in barley

Project description:ABA INSENSITIVE 5 (ABI5) is a basic leucine zipper (bZIP) transcription factor which acts in the abscisic acid (ABA) signaling and is activated in response to abiotic stresses. It was shown that ABI5 binds ABA RESPONSIVE ELEMENTs (ABRE cis-elements) present in the promoters of regulated genes and activates or represses their transcription in response to stress. However, the precise role of barley (Hordeum vulgare) ABI5 in ABA signaling is still not well understood. We have identified a hvabi5.d mutant using barley TILLING (Targeted Induced Local Lesions IN Genomes) platform. hvabi5.d showed drought tolerant phenotype. To identify molecular mechanisms responsible for hvabi5.d response to drought, we perform drought-related gene expression analysis in barley in two genotypes: the wild-type (WT) barley cultivar 'Sebastian’ and hvabi5.d mutant; in two time points: (1) optimal water conditions, and (2) after 10 days of drought stress in the second leaf; analyses were performed in three biological replicates. Global transcriptome analysis (Agilent Barley Microarray) of the mutant and parent cultivar ‘Sebastian’ exposed to drought enabled to identify genes in hvabi5.d which were associated with better response of the mutant to drought. These data increase our understanding of HvABI5-dependent modulation of plant response to the drought stress.

2020-07-22 | E-MTAB-9072 | biostudies-arrayexpress

Hybrid aspen (Populus tremula x tremuloides) cambial zone RNA-sequencing with 35um wide fraction scheme

Project description:Aim of the project: Genome wide gene expression profiles across the cambial zone are analyzed in 35um resolution from wild type hybrid aspen (Populus tremula x tremuloides) and two independent LMX5::AtIPT7 over expressor transgenic Populus tree lines.

2016-07-14 | E-MTAB-4631 | biostudies-arrayexpress

Populus tremula x Populus tremuloides

Project description:Populus tremula x Populus tremuloides EST project

| PRJNA13256 | ENA