Project description:We profiled the DNA methylation of saliva cell types, to develop a tool for epidemiologic studies. Saliva was collected from 22 children, 21 participants with samples usable for DNA methylation, and sorted into immune and epithelial cells, using size exclusion filtration and magnetic bead sorting. DNA methylation was measured using the Illumina MethylationEPIC BeadChip. Saliva immune and epithelial cells have distinct DNA methylation profiles, which can influence whole saliva epidemiologic measures.
Project description:This study aimed to perform a genomic and epidemiology analysis on Legionella isolates to determine species and strain distribution in hospital environment according to isolation date, treatment employed to eradicate the bacteria, and place of isolation. We sequenced the whole genome of 101 isolates of Legionella pneumophila and 12 Legionella rubrilucens.
