Project description:We used RNA-seq to profile gene expression changes during flg22 activated pattern-triggered immunity in multiple Brassicaceae including Capsella rubella, Cardamine hirsuta and Eutrema salsugineum as well as in multiple Arabidopsis thaliana accessions. This allows comparative transcriptomics within and across species to investigate the evolution of stress-responsive transcrption changes in these species.
Project description:Plant cells employ cell-surface receptors to detect pathogens and launch immunity.Here we show that flg22 can induce a receptor-like kinase CERK1 phosphorylation at juxtamembrane region (JM). However, Genome-wide transcriptional reprogramming and other flg22 responses implied that CERK1 does not directly mediate flg22 signaling. Surprisingly, CERK1 JM phosphorylation can promote chitin responses and fungal resistance for plants and genome-wide transcriptional reprogramming also suggested no sign of constitutive immunity caused by this.
