Project description:Propionibacterium freudenreichii is an important starter culture used in the manufacture of Swiss-type cheeses. We have generated the complete genome sequence of a Propionibacterium freudenreichii ssp. shermanii strain JS at the Institute of Biotechnology, University of Helsinki, by using a combination of pyrosequencing with GS FLX and GS FLX Titanium series reagents (Roche) and SOLiD 4 (Life Technologies), ABI 3130xl Genetic Analyzer (Life Technologies), and PacBio RS II (Pacific Biosciences) instruments. Initial genome annotation was carried out using RAST, and additional functional annotation information for each CDS was obtained from BLANNOTATOR, CDD, and KAAS. Accession number for genome sequence is PRJEB12148. This submission is for the transcriptome analysis of Propionibakcterium freudenreichii in cheese ripening under warm and cold conditions. The RNA reads were mapped to the reference genome PRJEB12148.
Project description:This study was conducted to investigate whether 1,2-propanediol could be metabolized by Propionibacterium freudenreichii DSM 20271 through bacterial microcompartment mediated metabolism.
Project description:We reported that analysis of acid tolerance mechanism in Propionibacterium acidipropionici at gene expression level. Genome shuffling was performed on P. acidipropionici to improve the acid tolerance of the strain. Wild type strain P. acidipropionici CGMCC 1.2230 and acid-tolerant mutant P. acidipropionici WSH1105 were compared to find key genes involved in acid tolerance of P. acidipropionici during fermentation. Results provide important information on acid response of P. acidipropionici, such as genes encoded ABC-transporters, proteins involved in amino acids metabolism and propionic acid synthesis.
