ABSTRACT: Effect of co-digestion of laundry wastewater and domestic sewage on microbial community resilience regarding sulfide production in a pilot-scale anaerobic reactor
Project description:Anaerobic co-digestion of commercial laundry wastewater and domestic sewage in EGSG pilot scale reactor: influence of surfactant concentration on microbial diversity, evaluation of microbial stratification and bamA gene for anaerobic ring fission
| PRJEB19914 | ENA
Project description:Metagenome of pilot-scale anaerobic digestion of sewage sludge
Project description:This experiment was performed to help optimize a digestion protocol for FACS of adult mouse lung cells. The sample JH2 was used to help generate lung epithelial cell signatures.
Project description:The structural complexity of nucleosomes underlies their functional versatility. Here we report a new type of complexity – nucleosome fragility, manifested as high sensitivity to micrococcal nuclease, in contrast to the common presumption that nucleosomes are similar in resistance to MNase digestion. Using differential MNase digestion of chromatin and high-throughput sequencing, we have identified a special group of nucleosomes termed fragile nucleosomes throughout the yeast genome, nearly one thousand of which are at previously determined “nucleosome free” loci. Nucleosome fragility is broadly implicated in multiple chromatin processes, including transcription, translocation and replication, in correspondence to specific physiological states of cells. In the environmental-stress-response genes, the presence of fragile nucleosomes prior to the occurrence of environmental changes suggests that nucleosome fragility poises genes for swift up-regulation in response to the environmental changes. We propose that nucleosome fragility underscores distinct functional statuses of the chromatin and provides a new dimension for portraying the landscape of genome organization. Comparing nucleosome occupancy under different MNase digestion levels and growth conditions.
