Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Metatranscriptomics of socially defeated mice

ABSTRACT: Metatranscriptomics of socially defeated mice

PROVIDER: PRJEB32472 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Similar Datasets

Metatranscriptomics of socially defeated mice

Project description:To address the mechanism underlying the immun-modulation of T cells in socially defeated mice, we wondered whether microbiota transcribe new virulent genes that challenge the murine immune system. So, full meta-transcriptomics was conducted of these 2 groups of mice.

2019-05-09 | E-MTAB-7943 | biostudies-arrayexpress

Wheat rhizosphere metatranscriptomics

Project description:Wheat rhizosphere metatranscriptomics

| PRJEB2451 | ENA

Kentrophoros metatranscriptomics

Project description:Metatranscriptomics of Kentrophoros ciliate-bacteria symbiosis

| PRJEB25540 | ENA

post-wash metatranscriptomics project

Project description:post-wash metatranscriptomics project

| PRJEB45608 | ENA

Genome-resolved metatranscriptomics reveals conserved root colonization determinants in a synthetic microbiota

Project description:The identification of processes activated by specific microbes during microbiota colonization of plant roots has been hampered by technical constraints in metatranscriptomics. These include lack of reference genomes, high representation of host or microbial rRNA sequences in datasets, or difficulty to experimentally validate gene functions. Here, we recolonized germ-free Arabidopsis thaliana with a synthetic, yet representative root microbiota comprising 106 genome-sequenced bacterial and fungal isolates. We used multi-kingdom rRNA depletion, deep RNA-sequencing and read mapping against reference microbial genomes to analyse the in-planta metatranscriptome of abundant colonizers. We identified over 3,000 microbial genes that were differentially regulated at the soil-root interface. Translation and energy production processes were consistently activated in planta, and their induction correlated with bacterial strains’ abundance in roots. Finally, we used targeted mutagenesis to show that several genes consistently induced by multiple bacteria are required for root colonization in one of the abundant bacterial strains (a genetically tractable Rhodanobacter). Our results indicate that microbiota members activate strain-specific processes but also common gene sets to colonize plant roots.

2023-11-03 | GSE231841 | GEO

Transcriptome analysis of the kidney of socially stressed male C57 BL/6 mice

Project description:To investigate the difference of gene expression between kidneys of groups with different treatments, We performed gene expression profiling analysis using data obtained from RNA-seq of kidney from socially stressed male C57 BL/6 mice with different treatments.

2023-09-15 | GSE207710 | GEO

Metatranscriptomics libraries - Whalers Bay

Project description:Metatranscriptomics libraries - Whalers Bay - Deception Island - Maritime Antarctic

| PRJEB42062 | ENA

Fecal metatranscriptomics from healthy adults before and after supplementation with bovine milk oligosaccharides

Project description:Complex oligosaccharides found in human milk play a vital role in gut microbiome development for the human infant. Bovine milk oligosaccharides (BMO) have similar structures with those derived from human milk, but have not been well studied for their effects on the healthy adult human gut microbiome. Healthy human subjects consumed BMO over two-week periods at two different doses and provided fecal samples. Metatranscriptomics of fecal samples was conducted to determine microbial and host gene expression in response to the supplement. Fecal samples were also analyzed by mass spectrometry to determine levels of undigested BMO. No changes were observed in microbiome activity across all participants. Repeated sampling enabled subject-specific analyses: four of six participants had minor, yet statistically significant, changes in microbial activity. No significant change was observed in the gene expression of host cells in stool. Levels of BMO excreted in feces after supplementation were not significantly different from placebo and were not correlated with dosage or expressed microbial enzyme levels. Collectively, these data suggest that BMO is fully digested in the human gastrointestinal tract prior to stool collection. Participants’ gut microbiomes remained stable but varied between individuals. Additionally, the unaltered host transcriptome provides further evidence for the safety of BMO as a dietary supplement or food ingredient.

2020-02-18 | GSE108809 | GEO

Metagenomics/Metatranscriptomics of shallow, oligotrophic aquifers

Project description:Metagenomics/Metatranscriptomics of the Hainich CZE

| PRJEB28738 | ENA

Metagenomics-Metatranscriptomics of Tarim solis

Project description:Metagenomics and Metatranscriptomics of microbial community in Tarim solis

| PRJEB21269 | ENA