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PUF3 RNAi in bloodstream-form of Trypanosoma brucei

ABSTRACT: PUF3 RNAi in bloodstream-form of Trypanosoma brucei

PROVIDER: PRJEB33062 | ENA |

REPOSITORIES: ENA

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PUF3 RNAi in bloodstream-form trypanosomes

Project description:PUF3 was depleted by RNAi for 24h in bloodstream-form trypanosomes (Trypanosoma brucei). No effect was seen on the transcriptome.

2019-06-30 | E-MTAB-8062 | biostudies-arrayexpress

Pull-down of PTP-tagged EIF4E2 from bloodstream-form Trypanosoma brucei

Project description:Pull-down of PTP-tagged EIF4E2 from bloodstream-form Trypanosoma brucei, sequence of bound and unbound fractions

2021-08-31 | E-MTAB-10670 | biostudies-arrayexpress

TbSAP is a novel chromatin protein repressing metacyclic Variant Surface Glycoprotein expression sites in bloodstream form Trypanosoma brucei

Project description:The African trypanosome Trypanosoma brucei is a unicellular eukaryote, which relies on a protective Variant Surface Glycoprotein (VSG) coat for survival in the mammalian host. A single trypanosome has >2000 VSG genes and pseudogenes of which only one is expressed from one of ~15 telomeric bloodstream form expression sites (BESs). Infectious metacyclic trypanosomes present within the tsetse fly vector also express VSG from a separate set of telomeric metacyclic ESs (MESs). All MESs are silenced in bloodstream form T. brucei. As very little is known about how this is mediated, we performed a whole genome RNAi library screen to identify MES repressors. This allowed us to identify a novel SAP domain containing DNA binding protein which we called TbSAP. TbSAP is enriched at the nuclear periphery and binds both MESs and BESs. Knockdown of TbSAP in bloodstream form trypanosomes did not result in cells becoming more ‘metacyclic’-like. Instead, there was extensive global upregulation of transcripts including MES VSGs, VSGs within the silent VSG arrays as well as genes immediately downstream of BES promoters. TbSAP therefore appears to be a novel architectural chromatin protein playing an important role in silencing the extensive VSG repertoire of bloodstream form T. brucei.

2020-11-04 | GSE160713 | GEO

RNAi against DRBD18 in Trypanosoma brucei bloodstream forms

Project description:RNAi against DRBD18 in Trypanosoma brucei bloodstream forms

2021-06-29 | E-MTAB-9783 | biostudies-arrayexpress

mRNAs associated with EIF4E3 in bloodstream form T. brucei

Project description:mRNAs associated with EIF4E3 in bloodstream form T. brucei

2020-10-23 | E-MTAB-10665 | biostudies-arrayexpress

RNAi of XAC1 (Tb927.7.2780) in Trypanosoma brucei bloodstream forms

Project description:Gradient fractions of RNAi of XAC1 (Tb927.7.2780) in Trypanosoma brucei bloodstream forms. RNAi was induced using tetracycline and cell extracts were fractionated into polysomal and monosome-non-ribosome-associated fractions.

2017-12-16 | E-MTAB-6239 | biostudies-arrayexpress

Transcriptome sequencing of bloodstream form Trypanosoma brucei expressing VSG121

Project description:Transcriptome sequencing of bloodstream form Trypanosoma brucei expressing VSG121

| PRJEB19301 | ENA

RNAi of TbNMD3 in procyclic form Trypanosoma brucei lines

Project description:A procyclic form Trypansome brucei RNAi line (PTT parental line, transfected with pALC14 incorporating a TbNMD3 gene fragment) capable of inducing depletion of TbNMD3 was analysed for mRNA expression by RNAseq

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RNAseq of Trypanosoma brucei brucei EATRO1125 bloodstream forms without EIF4E2 compared with add-back

Project description:RNAseq of Trypanosoma brucei brucei EATRO1125 bloodstream forms without EIF4E2 compared with add-back

2021-08-31 | E-MTAB-10719 | biostudies-arrayexpress

Effect of ZC3H28 depletion (RNAi) in Trypanosoma brucei bloodstream forms

Project description:Bloodstream-form trypanosomes with inducible RNAi targeting ZC3H28 were incubated with the tetracycline inducer for 10h, cells without tetracycline were used as controls. This experiment was done in triplicates. In another similar experiment, bloodstream-form trypanosomes with inducible RNAi targeting ZC3H28 were incubated with the tetracycline inducer for 14h, 16h, and 24h. Cells without tetracycline (named ''no tet'' or ''0h'') were used as controls. This experiment was done in duplicates.

2021-07-31 | E-MTAB-10751 | biostudies-arrayexpress

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