Project description:It was found that after OA treatment, compared with the CK group, the abundance of lactic acid bacteria in the intestinal flora of mice in the OA group increased, and the increase in the abundance of lactic acid bacteria made the gene Il10 upregulated, Il10 had a significant effect on tumor volume reduction and prolongation of mouse survival, and played a role through cytokine receptor interaction pathway.
Project description:This experiment investigates yeast pathways involved in the secretion of metabolites that enable the growth of lactic acid bacteria. Wild-type Saccharomyces cerevisiae (S90) in different defined media, as well as four mutant yeasts with selective gene deletions, were forwarded to RNA-seq to characterise global differences in transcriptomes and identify genes/pathways with altered gene expression levels.
Project description:Regulatory T cells (Tregs) are known to maintain survival and suppressive function in the presence of high levels of extracellular lactic acid. However, the effect of lactic acid on Treg induction is not known. We therfore evaluated the effect of lactic acid on Treg induction and observed an increased induction of Tregs in the presence of lactic acid. This increase occurred in a glycolysis-independent, acidity-dependent manner.
