Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

RNA-seq in differentiation of WT129 mESCs to neurons

ABSTRACT: RNA-seq in differentiation of WT129 mESCs to neurons

PROVIDER: PRJEB33696 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Similar Datasets

RNA-seq in differentiation of WT129 mESCs to neurons

Project description:Mouse WT129 ESCs were differentiated into glutamatergic neurons and samples were collected at days 0 (mESCs), 4 (embryoid bodies), 8 (neuronal precursors) and 12 (neurons). These are RNA-seq data to profile RNA expression during differentiation.

2020-05-21 | E-MTAB-8197 | biostudies-arrayexpress

ATAC-seq of mESCs differentiation into neurons

Project description:ATAC-seq of mESCs differentiation into neurons

| PRJEB33692 | ENA

ATAC-seq of mESCs differentiation into neurons

Project description:Mouse WT129 ESCs were differentiated into glutamatergic neurons and samples were collected at days 0 (mESCs), 4 (embryoid bodies), 8 (neuronal precursors) and 12 (neurons). ATAC-seq experiment in 4 biological replicates was performed at 4 indicated above time points to profile chromatin structure changes during differentiation.

2020-05-20 | E-MTAB-8194 | biostudies-arrayexpress

RNA-seq in TX1072 XO mESCs during differentiation

Project description:RNA-seq in TX1072 XO mESCs during differentiation

| PRJNA798741 | ENA

Sox2 ChIP-seq in mESCs and neurons

Project description:Mouse WT129 ESCs and differentiated from them within 12 days glutamatergic neurons were used for the ChiP-seq experiment with an antibody against Sox2 protein. ES stands for mESCs, NP for neurons, inputs are provided for both cell types.

2020-05-21 | E-MTAB-8196 | biostudies-arrayexpress

Sox2 ChIP-seq in mESCs and neurons

Project description:Sox2 ChIP-seq in mESCs and neurons

| PRJEB33695 | ENA

TT-seq and RNA-seq in TX1072 XXΔXic and XO mESCs during differentiation

Project description:TT-seq and RNA-seq in TX1072 XXΔXic and XO mESCs during differentiation

| PRJNA704378 | ENA

Quantitative Analysis of Wild Type(wt) and Setd2 knockout(ko) mESCs Transcriptomes [RNA-Seq]

Project description:Purpose: This study aimed at exploring the deregulated genes in setd2 knockout mESCs compared with wt, more particularly to find the mechanism controlled by setd2,which was required for endoderm differentiation. Methods: Setd2 wt and ko mESCs were generated by deep sequencing, using Illumina GAIIx. Using Avadis NGS (version:1.3) software to analyze the sequence reads that passed quality filter to acquire the expression level of all genes. qRT–PCR validation was performed usingSYBR Green assays. Results: Using an optimized data analysis workflow, we mapped about 80 million sequence reads per sample to the mouse genome (build mm9) and identified 17,827 transcripts in the sted2 wt and ko mESCs. About 2,516 genes were deregulated in setd2 ko mESCs, more than 10 genes were validated using qRT-PCR. Conclusions: Through RNA-seq,we noticed that a subset of genes that related to MAPK signaling pathways were down-regulated in ko mESCs. This provided a bridge to connect setd2 and mESCs endoderm differentiation. One wt and one ko mESCs were generated by deep sequencing, using Illumina GAIIx.

2014-10-14 | E-GEOD-54932 | biostudies-arrayexpress

STARR-seq in 1.8 XX and XO mESCs during differentiation

Project description:STARR-seq in 1.8 XX and XO mESCs during differentiation

| PRJNA704377 | ENA

ATAC-seq in TX1072 XXΔXic and XO mESCs during differentiation

Project description:ATAC-seq in TX1072 XXΔXic and XO mESCs during differentiation

| PRJNA704380 | ENA