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REPOSITORIES: ENA
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Project description:Metatranscriptomic Evidence for Direct Interspecies Electron Transfer Between Geobacter and Methanothrix Species in Rice Paddy Sediments
| PRJEB15510 | ENA
Project description:Geobacter species are of great interest for environmental and biotechnology applications as they can carry out direct electron transfer to insoluble metals or other microorganisms and have the ability to assimilate inorganic carbon. Here, we report on the capability and key enabling metabolic machinery of Geobacter metallireducens GS-15 to carry out CO2 fixation and direct electron transfer to iron. An updated metabolic reconstruction was generated, growth screens on targeted conditions of interest were performed, and constraint-based analysis was utilized to characterize and evaluate critical pathways and reactions in G. metallireducens. The novel capability of G. metallireducens to grow autotrophically with formate and Fe(III) was predicted and subsequently validated in vivo. Additionally, the energetic cost of transferring electrons to an external electron acceptor was determined through analysis of growth experiments carried out using three different electron acceptors (Fe(III), nitrate, and fumarate) by systematically isolating and examining different parts of the electron transport chain. The updated reconstruction will serve as a knowledgebase for understanding and engineering Geobacter and similar species.
2023-11-27 | MODEL2204190002 | BioModels
Project description:Transcriptomic analysis of interspecies electron transfer between Geobacter metallireducens and Geobacter sulfurreducens with the addition of three materials
| PRJNA383096 | ENA
Project description:A new model for electron flow during anaerobic digestion: direct interspecies electron transfer to Methanosaeta for the reduction of carbon dioxide to methane
| PRJEB4512 | ENA
Project description:The importance of the alternative route of electron donation to the plant ubiquinol pool via the electron-transfer flavoprotein/electron-transfer flavoprotein:ubiquinone oxidoreductase (ETF/ETFQO) complex has been well demonstrated. To assess the function of this alternative pathway therein, we performed a detailed transcription analyses of Arabidopsis mutants to investigate the molecular consequences of a dysfunctional ETF/ETFQO pathway. To this end, a comparative transcriptomic analysis of wild-type and two ETF/ETFQO complex mutants (etfqo, d2hgdh) was performed
2022-11-27 | E-MTAB-6207 | biostudies-arrayexpress