Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Gut microbiota of wild-living blackworm (V1-3 region, 454 pyrosequencing)

ABSTRACT: gut microbiota of wild-living blackworm (V1-3 region, 454 pyrosequencing)

PROVIDER: PRJEB34424 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Similar Datasets

gut microbiota of wild-living fish

Project description:gut microbiota of wild-living fish (V1-3 region, 454 pyrosequencing)

| PRJEB31232 | ENA

Microbial Community Succession on Steel – 454 pyrosequencing

Project description:In situ microbial community succession on mild steel in estuarine and marine environments – 454-pyrosequencing data

| PRJEB12866 | ENA

V1 vs V1 FlhA knockout

Project description:Competitive hybridization of V1 variant of genome sequenced C. jejuni strain 11168 versus V1 variant in which flhA gene has been disrupted. Keywords: repeat sample

2004-03-03 | GSE530 | GEO

V1 vs V1 FliA knockout

Project description:Competive hybridization of V1 variant of genome sequenced C. jejuni strain 11168 versus V1 variant in which fliA gene encoding sigma 28 has been disrupted Keywords: repeat sample

2004-03-03 | GSE521 | GEO

High-throughput 16S rRNA amplicon pyrosequencing of contaminated aquifer sediment

Project description:Contaminated aquifer (Dusseldorf-Flinger, Germany) templates extracted from 5 sediment depths ranging between 6.4 and 8.4 m below ground and over 3 years of sampling were amplified for amplicon pyrosequencing using the primers Ba27f (5’-aga gtt tga tcm tgg ctc ag-3’) and Ba519r (5’- tat tac cgc ggc kgc tg-3’), extended as amplicon fusion primers with respective primer A or B adapters, key sequence and multiplex identifiers (MID) as recommended by 454/Roche. Amplicons were purified and pooled as specified by the manufacturer. Emulsion PCR (emPCR), purification of DNA-enriched beads and sequencing run were performed following protocols and using a 2nd generation pyrosequencer (454 GS FLX Titanium, Roche) as recommended by the developer. Quality filtering of the pyrosequencing reads was performed using the automatic amplicon pipeline of the GS Run Processor (Roche), with a slight modification concerning the valley filter (vfScanAllFlows false instead of TiOnly) to extract the sequences. Demultiplexed raw reads were furhter trimmed for quality and lenght (>250 bp).

2012-11-11 | GSE25345 | GEO

High-throughput pyrosequencing of endogenous small RNAs from Solanum lycopersicum leaf and fruit

Project description:Comparison of the endogenous small RNA content of tomato leaves and fruits. Size fractionated small RNA from total RNA extracts was ligated to adapters, purified again and reverse transcribed. After PCR amplification the sample was subjected to 454 high throughput pyrosequencing. Please see www.454.com for details of the sequencing technology. Note: Raw data files were not available from 454 at the time this experiment was carried out.

2008-08-21 | E-GEOD-12081 | biostudies-arrayexpress

High-throughput pyrosequencing of endogenous small RNAs from Arabidopsis thaliana wild-type and polIV mutants

Project description:Comparison of the endogenous small RNA content of Arabidopsis flower bud tissue: wild type vs. mutants in polIV pathways Keywords: High throughput 454 small RNA sequencing. Size fractionated small RNA from total RNA extracts was ligated to adapters, purified again and reverse transcribed. After PCR amplification the sample was subjected to 454 high throughput pyrosequencing. Please see www.454.com for details of the sequencing technology.

2008-02-25 | E-GEOD-10180 | biostudies-arrayexpress

High-throughput 16S rRNA amplicon pyrosequencing of contaminated aquifer sediment

2012-11-11 | E-GEOD-25345 | biostudies-arrayexpress

Pyrosequencing based on cpcBA-IGS

Project description:Cyanobacterial community composition in eutrophic lakes by 454 pyrosequencing

| PRJEB15368 | ENA

Streptococcus sp. v1 strain:v1

Project description:Streptococcus sp. v1 strain:v1 Genome sequencing

| PRJNA658170 | ENA