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Bacteroides thetaiotaomicron TS7.3-1.3

ABSTRACT: Strain Bacteroides thetaiotaomicron TS7.3-1.3 (species Bacteroides thetaiotaomicron) was isolated from the fecal microbiota of a USA female at time point zero (bacterial isolates were sequenced from this donor on day 0, 364, 399, and 484). The species name was assigned by genome clustering.

PROVIDER: PRJEB3447 | ENA |

REPOSITORIES: ENA

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Bacteroides thetaiotaomicron TS7.3-1.5

Project description:Strain Bacteroides thetaiotaomicron TS7.3-1.5 (species Bacteroides thetaiotaomicron) was isolated from the fecal microbiota of a USA female at time point zero (bacterial isolates were sequenced from this donor on day 0, 364, 399, and 484). The species name was assigned by genome clustering.

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Project description:Strain Bacteroides thetaiotaomicron TS7.3-1.1 (species Bacteroides thetaiotaomicron) was isolated from the fecal microbiota of a USA female at time point zero (bacterial isolates were sequenced from this donor on day 0, 364, 399, and 484). The species name was assigned by genome clustering.

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Project description:Strain Bacteroides thetaiotaomicron TS7.3-1.4 (species Bacteroides thetaiotaomicron) was isolated from the fecal microbiota of a USA female at time point zero (bacterial isolates were sequenced from this donor on day 0, 364, 399, and 484). The species name was assigned by genome clustering.

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Bifidobacterium longum TS7.3-1.3

Project description:Strain Bifidobacterium longum TS7.3-1.3 (species Bifidobacterium longum) was isolated from the fecal microbiota of a USA female at time point zero (bacterial isolates were sequenced from this donor on day 0, 364, 399, and 484). The species name was assigned by genome clustering.

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Project description:Strain Coprococcus comes TS7.3-1.3 (species Coprococcus comes) was isolated from the fecal microbiota of a USA female at time point zero (bacterial isolates were sequenced from this donor on day 0, 364, 399, and 484). The species name was assigned by genome clustering.

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Project description:Strain Bacteroides thetaiotaomicron TSDC20.2-1.3 (species Bacteroides thetaiotaomicron) was isolated from the fecal microbiota of a USA female at time point zero (bacterial isolates were sequenced from this donor on day 0 and 57). The species name was assigned by genome clustering.

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Depolymerization of plant cell wall glycans by symbiotic human gut bacteria (Bacteroides thetaiotaomicron)

Project description:Symbiotic bacteria inhabiting the distal human gut have evolved under intense pressure to utilize complex carbohydrates, predominantly plant cell wall glycans abundant in our diets. These substrates are recalcitrant to depolymerization by digestive enzymes encoded in the human genome, but are efficiently targeted by some of the ~103-104 bacterial species that inhabit this niche. These species augment our comparatively narrow carbohydrate digestive capacity by unlocking otherwise unusable sugars and fermenting them into host-absorbable forms, such as short-chain fatty acids. We used phenotype profiling, whole-genome transcriptional analysis and molecular genetic approaches to investigate complex glycan utilization by two fully sequenced and closely related human gut symbionts: Bacteroides thetaiotaomicron and Bacteroides ovatus. Together these species target all of the common glycosidic linkages found in the plant cell wall, as well as host polysaccharides, but each species exhibits a unique ‘glycan niche’: in vitro B. thetaiotaomicron targets plant cell wall pectins in addition to linkages contained in host N- and O-glycans; B. ovatus uniquely targets hemicellulosic polysaccharides along with several pectins, but is deficient in host glycan utilization. Growth of Bacteroides thetaiotaomicron in vitro in minimal medium plus different purified complex glycans. Observation of increased gene expression was used to determine genes that are involved in metabolism of each glycan. Two biological replicates each.

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