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Transcriptomic analysis of mouse splenic B cells that are either TG2-sufficient or TG2-deficient

ABSTRACT: Transcriptomic analysis of mouse splenic B cells that are either TG2-sufficient or TG2-deficient

PROVIDER: PRJEB35258 | ENA |

REPOSITORIES: ENA

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Transcriptomic analysis of mouse splenic B cells that are either TG2-sufficient or TG2-deficient

Project description:To compare the transcriptomes of TG2-sufficient and TG2-deficient mouse splenic B cells under resting and stimulated conditions.

2019-11-08 | E-MTAB-7567 | biostudies-arrayexpress

Transcriptional profile of thymic and splenic regulatory T cells, sufficient and deficient in miR-181a

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Single cell transcriptomic analysis (10x Genomics) of mouse splenic CD4+ T cells

Project description:Single cell transcriptomic analysis (10x Genomics) of mouse splenic CD4+ T cells

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Mouse thymic, splenic B cells and splenic DC immunopeptidome

Project description:C57BL/6 Mouse: I-Ab immunopeptidome presented by thymus, splenic B cells and splenic DCs. Human lymphoblastoid cells: HLA-DR immunopeptidome identified from different cell dilutions. Splenic B cells Files- B cells 1 (121616WTII), B cells 2 (072417MHCII-1), B cells 3(072417MHCII-2) Thymus Files-Thymus 1 (120116WTII), Thymus 2 (020617WT MHCII), Thymus 3 (061417MHCII WT-1), Thymus 4 (061417MHCII WT-2) Splenic DCs- DC1 (021517 Immature), DC2 (021517 Mature), DC3 (022018_BL6_Control), DC4 (022018_BL6_FLT3)

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Microarray data for Bhlhe40-sufficient and -deficient resident macrophages

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Ex-vivo transcriptional analysis of splenic autophagy deficient dendritic cell subsets in an atherogenesis mouse model

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Expression data from retinoic acid-sufficient and retinoic acid-deficient mouse airway smooth muscle [mouse ASMs]

Project description:Observational studies in human suggest involvement of vitamin A/retinoic acid (RA) signaling in the regulation of airway smooth muscle (ASM) function, but the precise mechanisms by which RA impacts ASM phenotype is not clear. Here, we generated trascriptional profiles from two different models of RA-sufficient and RA-deficient mouse ASM in order to determine the molecular targets of RA in ASM (VAS/VAD, CTR/BMS) Mouse ASMs were isolated from Acta2-hrGFP;Cspg4-DsRED adult mice using flow cytometry. VAS mice were given normal, vitamin A-sufficient diet (15 IU vitamin/g) while the VAD mice were given 3 days of vitamin A-deficient diet (< 0.1 IU vitamin/g). CTR/BMS mouse ASMs were also isolated from Acta2-hrGFP;Cspg4-DsRED adult mice using flow cytometry. CTR mice were given 3 days of powdered, vitamin A sufficient diet mixed with vehicles (corn oil) while the BMS diet were given 3 days of powdered, vitamin A sufficient diet mixed with a potent pan-RA-receptor antagonist (BMS, 5 mcg/g).

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RNA-seq analysis of transcriptomes of Noc4L deficient and the Nco4L sufficient Treg cells

Project description:To gain in depth transcriptional information about the Noc4L deficient and Noc4L sufficient Tregs, we performed RNA-seq analysis to compare the transcriptomes of Noc4L deficient (CD4+GFP+CD62Lhigh, cKO) and Noc4L sufficient (CD4+GFP+CD62Lhigh, Het) Tregs activated in vitro with Dynabeads for 48 hours or 72 hours. We found ~25 unique genes with differential expression between the Noc4L deficient and Noc4L sufficient Treg cells when activated for 48 hours in vitro. And ~100 unique genes with differential expression between the Noc4L deficient and Noc4L sufficient Treg cells when activated for 72 hours in vitro in two times experiment.

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Project description:We analyzed the transcriptome differences of wild-type and ArhGEF1-deficient (Arhgef1-/-) type-2 conventional dendritic cells (cDC2s) in spleen. Wild-type or ArhGEF1-deficient (Arhgef1-/-) bone marrow cells were transferred into lethal irradiated CD45.1 mice. 8 weeks later, splenic cDC2s were purified and prepared for RNA-seq analysis. The gene expression profiles of CD97-, Gα13- and ArhGEF1-deficient cDC2s were highly similar, providing evidence that these molecules function in the same pathway.

2022-03-23 | GSE188833 | GEO

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